Paralytic shellfish toxin producing Aphanizomenon gracile strains isolated from Lake Iznik, Turkey

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dc.authorid0000-0002-0982-727Xen_US
dc.contributor.authorYılmaz, Mete
dc.contributor.authorFoss, Amanda J.
dc.contributor.authorSelwood, Andrew I.
dc.contributor.authorOzen, Mihriban
dc.contributor.authorBoundy, Michael
dc.date.accessioned2021-03-20T20:13:12Z
dc.date.available2021-03-20T20:13:12Z
dc.date.issued2018
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.descriptionSelwood, Andrew/0000-0003-1399-8028; Yilmaz, Mete/0000-0002-0982-727X; Foss, Amanda/0000-0003-0412-3879en_US
dc.description.abstractAphanizomenon gracile is one of the most widespread Paralytic Shellfish Toxin (PST) producing cyanobacteria in freshwater bodies in the Northern Hemisphere. It has been shown to produce various PST congeners, including saxitoxin (STX), neosaxitoxin (NEO), decarbamoylsaxitoxin (dcSTX) and gonyautoxin 5 (GTX5) in Europe, North America and Asia. Three cyanobacteria strains were isolated in Lake Iznik in northwestern Turkey. Morphological characterization of these strains suggested all three strains conformed to classical taxonomic identification of A. gracile with some differences such as clumping of filaments, partially hyaline cells in some filaments and longer than usual vegetative cells. Sequences of 16S rRNA gene of these strains were placed within an A. gracile cluster including the majority of PST producing strains, confirming the identification of these strains as A. gracile. These new strains possessed saxitoxin biosynthesis genes sxtA, sxtG and their sequences clustered with those of other A. gracile. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis demonstrated the presence of NEO, STX, dcSTX and decarbamoylneosaxitoxin (dcNEO) in all strains. This is the first report of a PST producer in any water body in Turkey and first observation of dcNEO in an A. gracile culture. (C) 2018 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific and Technologic Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Y641]en_US
dc.description.sponsorshipThis study was supported by indirect costs of a grant from The Scientific and Technologic Research Council of Turkey (TUBITAK) given to Mete Yilmaz (Project no: 114Y641). MY performed the taxonomic and phylogenetic analyses. AJF, AIS and MB performed the LC-MS/MS analyses. MO isolated the strains and performed the PCRs. MY wrote the paper, AJF and AIS contributed to writing. Authors thank two anonymous reviewers for critically reading the manuscript and for valuable suggestions.en_US
dc.identifier.doi10.1016/j.toxicon.2018.04.028en_US
dc.identifier.endpage142en_US
dc.identifier.issn0041-0101
dc.identifier.pmid29705145en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage132en_US
dc.identifier.urihttp://doi.org/10.1016/j.toxicon.2018.04.028
dc.identifier.urihttps://hdl.handle.net/20.500.12885/815
dc.identifier.volume148en_US
dc.identifier.wosWOS:000434745300016en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Mete
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofToxiconen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAphanizomenon gracileen_US
dc.subjectDecarbamoylneosaxitoxinen_US
dc.subjectLake Izniken_US
dc.subjectNeosaxitoxinen_US
dc.subjectParalytic shellfish toxinen_US
dc.subjectSaxitoxinen_US
dc.titleParalytic shellfish toxin producing Aphanizomenon gracile strains isolated from Lake Iznik, Turkeyen_US
dc.typeArticleen_US

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