An alginate-poly(acrylamide) hydrogel with TGF-?3 loaded nanoparticles for cartilage repair: Biodegradability, biocompatibility and protein adsorption

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dc.authorid0000-0002-8947-8494en_US
dc.contributor.authorSaygili, E.
dc.contributor.authorKaya, E.
dc.contributor.authorIlhan-Ayisigi, E.
dc.contributor.authorSaglam-Metiner, P.
dc.contributor.authorAlarcin, E.
dc.contributor.authorKazan, Aslıhan
dc.contributor.authorGirgic, E.
dc.date.accessioned2021-03-20T20:26:52Z
dc.date.available2021-03-20T20:26:52Z
dc.date.issued2021
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.descriptionPubMed ID: 33476613en_US
dc.description.abstractCurrent implantable materials are limited in terms of function as native tissue, and there is still no effective clinical treatment to restore articular impairments. Hereby, a functionalized polyacrylamide (PAAm)-alginate (Alg) Double Network (DN) hydrogel acting as an articular-like tissue is developed. These hydrogels sustain their mechanical stability under different temperature (+4 °C, 25 °C, 40 °C) and humidity conditions (60% and 75%) over 3 months. As for the functionalization, transforming growth factor beta-3 (TGF-?3) encapsulated (NPTGF-?3) and empty poly(lactide-co-glycolide) (PLGA) nanoparticles (PLGA NPs) are synthesized by using microfluidic platform, wherein the mean particle sizes are determined as 81.44 ± 9.2 nm and 126 ± 4.52 nm with very low polydispersity indexes (PDI) of 0.194 and 0.137, respectively. Functionalization process of PAAm-Alg hydrogels with ester-end PLGA NPs is confirmed by FTIR analysis, and higher viscoelasticity is obtained for functionalized hydrogels. Moreover, cartilage regeneration capability of these hydrogels is evaluated with in vitro and in vivo experiments. Compared with the PAAm-Alg hydrogels, functionalized formulations exhibit a better cell viability. Histological staining, and score distribution confirmed that proposed hydrogels significantly enhance regeneration of cartilage in rats due to stable hydrogel matrix and controlled release of TGF-?3. These findings demonstrated that PAAm-Alg hydrogels showed potential for cartilage repair and clinical application. © 2021 Elsevier B.V.en_US
dc.description.sponsorship117M843 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK National Research Foundation of Korea, NRF: 2017K2A9A1A06037807en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under grant number 117M843 . Y.-W.Kim and J.-Y.Sun were supported under the framework of international cooperation program managed by the National Research Foundation of Korea ( 2017K2A9A1A06037807 , FY2017 ).en_US
dc.identifier.doi10.1016/j.ijbiomac.2021.01.069en_US
dc.identifier.endpage393en_US
dc.identifier.issn0141-8130
dc.identifier.scopus2-s2.0-85099620615en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage381en_US
dc.identifier.urihttp://doi.org/10.1016/j.ijbiomac.2021.01.069
dc.identifier.urihttps://hdl.handle.net/20.500.12885/1307
dc.identifier.volume172en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKazan, Aslıhan
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlginateen_US
dc.subjectArticular cartilageen_US
dc.subjectDouble network hydrogelsen_US
dc.subjectGrowth factoren_US
dc.subjectNanoparticlesen_US
dc.titleAn alginate-poly(acrylamide) hydrogel with TGF-?3 loaded nanoparticles for cartilage repair: Biodegradability, biocompatibility and protein adsorptionen_US
dc.typeArticleen_US

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