Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

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dc.authorid0000-0002-0290-1166en_US
dc.contributor.authorAydin, Sevtap
dc.contributor.authorSahin, Tevfik Tolga
dc.contributor.authorBacanli, Merve
dc.contributor.authorTaner, Gökçe
dc.contributor.authorBasaran, Arif Ahmet
dc.contributor.authorAydin, Mehtap
dc.date.accessioned2021-03-20T20:14:22Z
dc.date.available2021-03-20T20:14:22Z
dc.date.issued2016
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.description.abstractBackground: The increases of free radicals have been proposed to be involved in the pathogenesis of sepsis, which leads to multiple-organ dysfunction syndromes. The uses of antioxidants as a complementary tool in the medical care of oxidative stress-related diseases have attracted attention of researchers. Resveratrol (RV) has suggested being antioxidant, anti-proliferative, and anti-inflammatory effects in various experimental models and clinical settings. Aims: This study was undertaken to evaluate the protective effects of RV on oxidative DNA damage induced by sepsis in the liver and kidney tissues of Wistar albino rats. Study Design: Animal experimentation. Methods: Four experimental groups consisting of eight animals for each was created using a total of thirty-two male Wistar albino rats. Sham group was given 0.5 mL of saline intra-peritoneal (ip) only following laparatomy. Sepsis group was given 0.5 mL saline ip only following the induction of sepsis. RV-treated group was given a dose of 100 mg/kg ip RV in 0.5 mL saline following laparatomy. RV-treated sepsis group was given 100 mg/kg ip RV in 0.5 mL saline following the induction of sepsis. A model of sepsis was created by cecal ligation and puncture technique. In the liver and kidney tissues, oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX)) and a proinflammatory cytokine (tumor necrosis factor alpha (TNF-alpha)), were evaluated spectrophotometrically and DNA damage was determined by the alkaline single cell gel electrophoresis (comet assay) technique using formamidopyrimidine DNA glycosylase protein. Results: In the RV-treated sepsis group, the levels of MDA and TNF-alpha were lower and GSH levels, SOD and GPX activities were higher than in the septic rats (p<0.05). RV treatment significantly reduced the sepsis-induced oxidative DNA damage in the liver and kidney cells (p<0.05). Conclusion: It is suggested that RV treatment might reduce the sepsis-induced oxidative DNA damages in sepsis-related diseases; however, there is a need for more studies to clear up the protective mechanisms of RV against sepsis.en_US
dc.identifier.doi10.5152/balkanmedj.2016.15516en_US
dc.identifier.endpage601en_US
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue6en_US
dc.identifier.pmid27994910en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage594en_US
dc.identifier.urihttp://doi.org/10.5152/balkanmedj.2016.15516
dc.identifier.urihttps://hdl.handle.net/20.500.12885/1042
dc.identifier.volume33en_US
dc.identifier.wosWOS:000396360400002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTaner, Gökçe
dc.language.isoenen_US
dc.publisherGalenos Yayinciliken_US
dc.relation.ispartofBalkan Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectResveratrolen_US
dc.subjectsepsisen_US
dc.subjecttumor necrosis factor alpha oxidative stressen_US
dc.subjectDNA damageen_US
dc.subjectalkaline single cell gel electrophoresisen_US
dc.titleResveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Ratsen_US
dc.typeArticleen_US

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