Assessment of DNA damage in welders using comet and micronucleus assays

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dc.authorid0000-0002-0290-1166en_US
dc.contributor.authorAksu, Ildeniz
dc.contributor.authorAnlar, Hatice Gul
dc.contributor.authorTaner, Gökçe
dc.contributor.authorBacanli, Merve
dc.contributor.authorIritas, Servet
dc.contributor.authorTutkun, Engin
dc.date.accessioned2021-03-20T20:12:34Z
dc.date.available2021-03-20T20:12:34Z
dc.date.issued2019
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.description.abstractWelding technology is widely used in pressurized containers, thermal power plants, refineries, chemical facilities and steel structures. Welders are exposed to a number of hazardous compounds such as ultraviolet (UV) radiation, electromagnetic fields, toxic metals and polycyclic aromatic hydrocarbons (PAHs). In the present study, 48 welders and an equal number of control subjects were evaluated for DNA damage in the whole blood and isolated lymphocytes using the comet assay. The genotoxic damage in buccal epithelial cells of subjects was determined by micronucleus (MN) assay. Metal(loids) such as Cr, Mn, Ni, Cu, As, Cd and Pb levels in blood samples were evaluated by using Inductively Coupled Plasma-Mass Spectrometer (ICP-MS). Results of this study showed that DNA damage in blood, isolated lymphocytes, and buccal epithelial cells were significantly higher in workers compared to the controls. Also, these workers had remarkably higher blood Cr, Cu, Cd, Ni and Pb levels. These results showed that occupational exposure to welding fumes may cause genotoxic damage that can lead to important health problems in the workers. More extensive epidemiological studies should be performed that enable the assessment of health risk in welding industry.en_US
dc.description.sponsorshipHacettepe University Scientific Research ProjectsHacettepe University [THD-2015-7282, THD-2017-15736]en_US
dc.description.sponsorshipThis work was supported by Hacettepe University Scientific Research Projects (Grant numbers: THD-2015-7282 and THD-2017-15736).en_US
dc.identifier.doi10.1016/j.mrgentox.2018.11.006en_US
dc.identifier.endpage45en_US
dc.identifier.issn1383-5718
dc.identifier.issn1879-3592
dc.identifier.pmid31421737en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage40en_US
dc.identifier.urihttp://doi.org/10.1016/j.mrgentox.2018.11.006
dc.identifier.urihttps://hdl.handle.net/20.500.12885/618
dc.identifier.volume843en_US
dc.identifier.wosWOS:000483453100007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTaner, Gökçe
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMutation Research-Genetic Toxicology And Environmental Mutagenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectWeldersen_US
dc.subjectWelding fumesen_US
dc.subjectAlkaline single cell gel electrophoresisen_US
dc.subjectBuccal micronucleus cytome assayen_US
dc.subjectInductively coupled plasma-mass spectrometeren_US
dc.titleAssessment of DNA damage in welders using comet and micronucleus assaysen_US
dc.typeArticleen_US

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