Highly selective and cost-effective impedimetric biosensor for cylindrospermopsin detection using an aptamer-functionalized pencil graphite electrode

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dc.authorid0000-0003-4616-5284
dc.authorid0000-0001-5807-633X
dc.contributor.authorKesici-Meco, Ece
dc.contributor.authorUnur-Yilmaz, Ece
dc.date.accessioned2026-02-08T15:15:29Z
dc.date.available2026-02-08T15:15:29Z
dc.date.issued2026
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractNutrient runoff from anthropogenic eutrophication, rising atmospheric CO2 levels, and global warming have significantly contributed to the proliferation of harmful cyanobacterial blooms in freshwater ecosystems. These blooms release cyanotoxins, such as cylindrospermopsin (CYN), which pose genotoxic and cytotoxic effects. The detection and monitoring of CYN in water are essential for protecting public health and reducing environmental risks. In this study, an electrochemical aptasensor was developed by effective immobilization of CYN-specific aptamer (cynApt) onto a highly conductive pencil graphite electrode (PGE), which offers a sustainable alternative to expensive gold and graphene-based sensors. CYN was detected through electrochemical impedance spectroscopy (EIS) by monitoring changes in charge transfer resistance. The detection limits for CYN using PGE/ cynApt electrodes were found to be 0.78 ng mL- 1 in deionized water and 0.97 ng mL- 1 in lake water, within a linear range of 1-20 ng mL- 1. The selectivity of the biosensor for CYN was also tested against other cyanotoxins, such as okadaic acid and saxitoxin, both in deionized and lake water.
dc.description.sponsorshipTUBITAK [2211/A, 124Z553]
dc.description.sponsorshipThis work was supported by TUBITAK (Project No: 124Z553) and the TUBITAK 2211/A and YOK 100/2000 PhD Scholarship Programs in Sustainable Agriculture.
dc.identifier.doi10.1016/j.toxicon.2025.108657
dc.identifier.issn0041-0101
dc.identifier.issn1879-3150
dc.identifier.pmid41224183
dc.identifier.scopus2-s2.0-105021509245
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.toxicon.2025.108657
dc.identifier.urihttps://hdl.handle.net/20.500.12885/5792
dc.identifier.volume269
dc.identifier.wosWOS:001621604700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofToxicon
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzWOS_KA_20260207
dc.subjectCylindrospermopsin
dc.subjectPencil graphite electrode
dc.subjectAptamer
dc.subjectElectrochemical biosensor
dc.subjectImpedimetric aptasensor
dc.titleHighly selective and cost-effective impedimetric biosensor for cylindrospermopsin detection using an aptamer-functionalized pencil graphite electrode
dc.typeArticle

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