Use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid

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dc.authorid0000-0002-0290-1166en_US
dc.contributor.authorTaner, Gökçe
dc.contributor.authorVardar, Deniz Ozkan
dc.contributor.authorAydin, Sevtap
dc.contributor.authorAytac, Zeki
dc.contributor.authorBasaran, Ahmet
dc.contributor.authorBasaran, Nursen
dc.date.accessioned2021-03-20T20:14:13Z
dc.date.available2021-03-20T20:14:13Z
dc.date.issued2017
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.description.abstractVanillic acid (VA) found in vanilla and cinnamic acid (CA) the precursor of flavonoids and found in cinnamon oil, are natural plant phenolic acids which are secondary aromatic plant products suggested to possess many physiological and pharmacological functions. In vitro and in vivo experiments have shown that phenolic acids exhibit powerful effects on biological responses by scavenging free radicals and eliciting antioxidant capacity. In the present study, we investigated the antioxidant capacity of VA and CA by the trolox equivalent antioxidant capacity (TEAC) assay, cytotoxicity by neutral red uptake (NRU) assay in Chinese Hamster Ovary (CHO) cells and also the genotoxic and antigenotoxic effects of these phenolic acids using the cytokinesis-blocked micronucleus (CBMN) and the alkaline comet assays in human peripheral blood lymphocytes. At all tested concentrations, VA (0.17-67.2 mu g/ml) showed antioxidant activity but CA (0.15-59.2 mu g/ml) did not show antioxidant activity against 2,2-azino-bis (3-ethylbenz-thiazoline-6-sulphonic acid) (ABTS). VA (0.84, 4.2, 8.4, 16.8, 84 and 168 mu g/ml) and CA (0.74, 3.7, 7.4, 14.8, 74, 148 mu g/ml) did not have cytotoxic and genotoxic effects alone at the studied concentrations as compared with the controls. Both VA and CA seem to decrease DNA damage induced by H2O2 in human lymphocytes.en_US
dc.description.sponsorshipGazi University Scientific Research Projects FoundationGazi University [05/2011-63]en_US
dc.description.sponsorshipThe authors declare that there are no conflicts of interest. This research was supported by the Gazi University Scientific Research Projects Foundation, 05/2011-63.en_US
dc.identifier.doi10.1080/01480545.2016.1190740en_US
dc.identifier.endpage190en_US
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue2en_US
dc.identifier.pmid27309403en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage183en_US
dc.identifier.urihttp://doi.org/10.1080/01480545.2016.1190740
dc.identifier.urihttps://hdl.handle.net/20.500.12885/1018
dc.identifier.volume40en_US
dc.identifier.wosWOS:000399742700008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTaner, Gökçe
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofDrug And Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVanillic aciden_US
dc.subjectcinnamic aciden_US
dc.subjecttrolox equivalent antioxidant capacityen_US
dc.subjectneutral red uptake assayen_US
dc.subjectcytokinesis-block micronucleus assayen_US
dc.subjectcomet assayen_US
dc.subjectCHO cellsen_US
dc.subjecthuman lymphocytesen_US
dc.titleUse of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic aciden_US
dc.typeArticleen_US

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