An enzyme immobilized microreactor for continuous-flow biocatalysis of ginsenoside Rb1

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dc.authorid0000-0002-8947-8494en_US
dc.contributor.authorKazan, Aslıhan
dc.contributor.authorHu X.
dc.contributor.authorStahl A.
dc.contributor.authorFrerichs H.
dc.contributor.authorSmirnova I.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2022-01-20T06:17:50Z
dc.date.available2022-01-20T06:17:50Z
dc.date.issued2021en_US
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.description.abstractGinsenoside Rb1 is one of the major bioactive components of Panax ginseng C.A. Meyer (Araliaceae), a medicinal plant that has been used for therapeutic purposes for thousands of years in Asian countries. The pharmaceutical activity of ginsenoside Rb1 highly depends on molecular structure and its deglycosylated metabolites are known to be more potent bioactive compounds. However, these deglycosylated ginsenosides do not exist naturally so they are usually obtained by poorly selective methods, like chemical hydrolysis. RESULTS: In this study, the development and characterization of an alginate-based immobilized enzyme microreactor for the catalytic conversion of ginsenoside Rb1 to more bioactive metabolites have been reported. Enzyme kinetic parameters were calculated and characterization tests (such as determination of surface area of alginate matrix, long-term use, and effect of residence time on conversion yield) were conducted. The system was operated under continuous-flow conditions and compared with acidic and batch enzymatic hydrolysis experiments, as conventional approaches. The enzymatic microreactor showed an enhanced activity by producing 13-fold higher amount of ginsenoside F2 than batch enzymatic hydrolysis. CONCLUSION: Obtained results indicated that the newly developed enzymatic microreactor could successfully convert ginsenoside Rb1 to more active metabolites and have a potential for the biocatalysis of multiple ginsenosides, as well as pharmaceutically active compounds.en_US
dc.identifier.endpage3357en_US
dc.identifier.issn02682575
dc.identifier.issue12en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage3349en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12885/1794
dc.identifier.volume96en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKazan, Aslıhan
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Ltden_US
dc.relation.isversionof10.1002/jctb.6887en_US
dc.relation.journalJournal of Chemical Technology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalginate hydrogelen_US
dc.subjectginsenosideen_US
dc.subjectimmobilizationen_US
dc.subjectmicroreactoren_US
dc.subjectβ-glucosidaseen_US
dc.titleAn enzyme immobilized microreactor for continuous-flow biocatalysis of ginsenoside Rb1en_US
dc.typeArticleen_US

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