Highly Transferable pAQU-Related Plasmids Encoding Multidrug Resistance Are Widespread in the Human and Fish Pathogen Photobacterium damselae subsp. damselae in Aquaculture Areas in the Black Sea

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dc.authorid0000-0002-5857-8820en_US
dc.contributor.authorVences, Ana
dc.contributor.authorAbushattal, Saqr
dc.contributor.authorMatanza, Xose M.
dc.contributor.authorDubert, Javier
dc.contributor.authorUzun, Ecren
dc.contributor.authorÖğüt, Hamdi
dc.date.accessioned2021-03-20T20:09:28Z
dc.date.available2021-03-20T20:09:28Z
dc.date.issued2020
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.description.abstractThe marine bacterium Photobacterium damselae subsp. damselae is a pathogen that causes disease in diverse marine animals, and is also a serious opportunistic human pathogen that can cause fatal infections. Strains of this pathogen isolated from diseased European sea bass in aquaculture facilities in the Turkish coast of the Black Sea were found to exhibit reduced sensitivity to multiple antimicrobials. Selected representative strains were subjected to complete genome sequencing and plasmid characterization. It was found that multidrug resistant (MDR) isolates harboured large conjugative plasmids sharing part of their sequence backbone with pAQU-group plasmids, hitherto reported exclusively in China and Japan. Four new pAQU-group versions of plasmids were identified in the present study, containing distinct combinations of the resistance determinants tetB, floR, sul2, qnrVC, dfrA and strAB. Conjugative transfer of pPHDD2-OG2, a representative plasmid of 170,998 bp, occurred at high frequencies (2.2 x 10(-2) transconjugants per donor cell), to E. coli and to pathogenic P. damselae subsp. damselae and subsp. piscicida strains. Upon transfer, pPHDD2-OG2 conferred reduced susceptibility to a number of antimicrobials to the recipient strains. Comparative genomics analysis of host strains suggested that these MDR plasmids of the pAQU-group were acquired by different genetic lineages of Pdd. This study provides evidence that P. damselae subsp. damselae isolated from diseased fish constitute a reservoir for conjugative MDR pAQU-group plasmids in the Mediterranean basin, and have the potential to spread to diverse bacterial species.en_US
dc.description.sponsorshipAgencia Estatal de Investigacion (AEI) of Spain - FEDER Programme from the European Union [AGL2016-79738-R]; Xunta de Galicia (Spain)Xunta de Galicia [ED431C 2018/18]en_US
dc.description.sponsorshipThis work was supported by the Agencia Estatal de Investigacion (AEI) of Spain co-funded by the FEDER Programme from the European Union (grant number AGL2016-79738-R), and by Xunta de Galicia (Spain) (grant number ED431C 2018/18).en_US
dc.identifier.doi10.1007/s00248-020-01519-4en_US
dc.identifier.endpage518en_US
dc.identifier.issn0095-3628
dc.identifier.issn1432-184X
dc.identifier.issue3en_US
dc.identifier.pmid32385615en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage507en_US
dc.identifier.urihttp://doi.org/10.1007/s00248-020-01519-4
dc.identifier.urihttps://hdl.handle.net/20.500.12885/429
dc.identifier.volume80en_US
dc.identifier.wosWOS:000531132300002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖğüt, Hamdi
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMicrobial Ecologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhotobacterium damselaeen_US
dc.subjectpAQUen_US
dc.subjectMDRen_US
dc.subjectPlasmiden_US
dc.subjectAcquired resistanceen_US
dc.subjectAquacultureen_US
dc.titleHighly Transferable pAQU-Related Plasmids Encoding Multidrug Resistance Are Widespread in the Human and Fish Pathogen Photobacterium damselae subsp. damselae in Aquaculture Areas in the Black Seaen_US
dc.typeArticleen_US

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