Electrochemical Investigation of DNA Interactions with Cyanobacterial Toxins: Cylindrospermopsin and Saxitoxin

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dc.authorid0000-0001-5807-633X
dc.authorid0000-0002-0982-727X
dc.contributor.authorKesici-Meco, Ece
dc.contributor.authorYilmaz, Mete
dc.contributor.authorUnur-Yilmaz, Ece
dc.date.accessioned2026-02-08T15:14:50Z
dc.date.available2026-02-08T15:14:50Z
dc.date.issued2025
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractAnthropogenic eutrophication threatens freshwater sources used for agricultural irrigation and increases the occurrence risk of cyanotoxins, such as cylindrospermopsin (CYN) and saxitoxin (STX). These toxins accumulate in surface waters and pose significant health risks, with CYN primarily affecting the liver and STX targeting the nervous system. In this study, dsDNA was immobilized on a pencil graphite electrode (PGE), and its interactions with CYN and STX were investigated by means of electrochemical impedance spectroscopy (EIS). Experimental conditions, including DNA concentration, cyanotoxin concentration, and DNA-cyanotoxin interaction time, were optimized to generate the most significant electrochemical signal (i.e., charge transfer resistance). The optimized PGE/DNA system established the foundation for an electrochemical biosensor that can be utilized for detection of cyanotoxins and detection limits of 0.12 ng mL-1 and 0.043 ng mL-1 were achieved for CYN and STX, respectively. The system was further analyzed using FTIR, UV-vis, and X-ray photoelectron spectroscopy (XPS), providing a comprehensive structural analysis of toxin-induced DNA alterations. The electrochemical system developed in this study by immobilizing DNA onto PGE allowed thorough investigation of the genotoxic effects of CYN and STX, as well as their sensitive detection.
dc.description.sponsorshipResearch Fund of the Bursa Technical University; TUBITAK 2211/A National PhD Scholarship Program; National PhD Scholarship Program in the Priority Fields in Sustainable Agriculture [YOK 100/2000]; [221N022]
dc.description.sponsorshipThis work was financially supported by Research Fund of the Bursa Technical University (Project Number: 221N022). The dissertation research is supported by the TUBITAK 2211/A National PhD Scholarship Program and the YOK 100/2000 National PhD Scholarship Program in the Priority Fields in Sustainable Agriculture.
dc.identifier.doi10.1002/slct.202405956
dc.identifier.issn2365-6549
dc.identifier.issue7
dc.identifier.scopus2-s2.0-85218980275
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1002/slct.202405956
dc.identifier.urihttps://hdl.handle.net/20.500.12885/5461
dc.identifier.volume10
dc.identifier.wosWOS:001425000600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistryselect
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzWOS_KA_20260207
dc.subjectCylindrospermopsin
dc.subjectDNA
dc.subjectElectrochemical biosensors
dc.subjectPencil graphite electrode
dc.subjectSaxitoxin
dc.titleElectrochemical Investigation of DNA Interactions with Cyanobacterial Toxins: Cylindrospermopsin and Saxitoxin
dc.typeArticle

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