Electrochemical Investigation of DNA Interactions with Cyanobacterial Toxins: Cylindrospermopsin and Saxitoxin
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Anthropogenic eutrophication threatens freshwater sources used for agricultural irrigation and increases the occurrence risk of cyanotoxins, such as cylindrospermopsin (CYN) and saxitoxin (STX). These toxins accumulate in surface waters and pose significant health risks, with CYN primarily affecting the liver and STX targeting the nervous system. In this study, dsDNA was immobilized on a pencil graphite electrode (PGE), and its interactions with CYN and STX were investigated by means of electrochemical impedance spectroscopy (EIS). Experimental conditions, including DNA concentration, cyanotoxin concentration, and DNA-cyanotoxin interaction time, were optimized to generate the most significant electrochemical signal (i.e., charge transfer resistance). The optimized PGE/DNA system established the foundation for an electrochemical biosensor that can be utilized for detection of cyanotoxins and detection limits of 0.12 ng mL-1 and 0.043 ng mL-1 were achieved for CYN and STX, respectively. The system was further analyzed using FTIR, UV-vis, and X-ray photoelectron spectroscopy (XPS), providing a comprehensive structural analysis of toxin-induced DNA alterations. The electrochemical system developed in this study by immobilizing DNA onto PGE allowed thorough investigation of the genotoxic effects of CYN and STX, as well as their sensitive detection.
Açıklama
Anahtar Kelimeler
Cylindrospermopsin, DNA, Electrochemical biosensors, Pencil graphite electrode, Saxitoxin
Kaynak
Chemistryselect
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
10
Sayı
7
