Cytotoxic and genotoxic effects of leaking chemicals from serum infusion sets: an in-vitro study

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dc.authorid0000-0002-0290-1166
dc.contributor.authorOzlu, Aysegul
dc.contributor.authorTaner, Gokce
dc.date.accessioned2026-02-12T21:05:32Z
dc.date.available2026-02-12T21:05:32Z
dc.date.issued2023
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractSafety concerns about medical devices playing important role in health sciences and bioengineering research are rising day by day. Although there are specific standards regarding disposable medical materials, the information is updating with the toxicological studies. In this study, cytotoxic/genotoxic effects of chemicals leaking from serum infusion sets that have an important place in the clinic were investigated. Media containing leakage chemicals were prepared from equal samples taken from the plastic line sections of 13 different brands of serum infusion sets containing phthalates and the effects on the cultured cells were compared with the untreated control groups. To obtain leaking chemicals, extracting period was selected as 72 h, a routine set-change time in the clinic as indicated in various publications. Neutral red uptake and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests were performed in L929 cells to determine cytotoxicity, and cytokinesis blocked micronucleus technique was performed in lymphocytes to determine genotoxicity. Cytotoxic and genotoxic damage levels were compared by evaluating cell-viability rates relative to control, micronucleus frequency, and nuclear division index values. The results showed that all sets caused a decrease in cell viability revealing the effects both on lysosomal and mitochondrial activity and increase in micronucleus frequencies in general. The number of similar studies is extremely limited, and in this study in addition to the short-term effects of using the serum infusion sets, the information about the sample tests to determine the biosecurity of disposable medical materials is given.
dc.identifier.doi10.1093/toxres/tfad010
dc.identifier.issn2045-452X
dc.identifier.issn2045-4538
dc.identifier.pmid37125335
dc.identifier.scopus2-s2.0-85166305073
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1093/toxres/tfad010
dc.identifier.urihttps://hdl.handle.net/20.500.12885/7017
dc.identifier.wosWOS:000940528600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.ispartofToxicology Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260212
dc.subjectserum infusion set
dc.subjectphthalates
dc.subjectcytotoxicity
dc.subjectgenotoxicity
dc.subjectmicronucleus test
dc.titleCytotoxic and genotoxic effects of leaking chemicals from serum infusion sets: an in-vitro study
dc.typeArticle

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