Comprehensive multi-technique approach reveals the high diversity of microcystins in field collections and an associated isolate of Microcystis aeruginosa from a Turkish lake
dc.authorid | 0000-0002-0982-727X | en_US |
dc.contributor.author | Yılmaz, Mete | |
dc.contributor.author | Foss, Amanda J. | |
dc.contributor.author | Miles, Christopher O. | |
dc.contributor.author | Ozen, Mihriban | |
dc.contributor.author | Demir, Nilsun | |
dc.contributor.author | Balcı, Muharrem | |
dc.date.accessioned | 2021-03-20T20:12:31Z | |
dc.date.available | 2021-03-20T20:12:31Z | |
dc.date.issued | 2019 | |
dc.department | BTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümü | en_US |
dc.description | Demir, Nilsun/0000-0002-3895-7655; BALCI, Muharrem/0000-0001-9373-6647; Foss, Amanda/0000-0003-0412-3879; Yilmaz, Mete/0000-0002-0982-727X; Miles, Chris/0000-0001-8537-4439; Beach, Daniel/0000-0002-5680-2112 | en_US |
dc.description.abstract | Microcystins (MCs) are hepatotoxic and potentially carcinogenic cyanotoxins. They exhibit high structural variability, with nearly 250 variants described to date. This variability can result in incomplete detection of MC variants during lake surveys due to the frequent use of targeted analytical methods and a lack of standards available for identification and quantitation. In this study, Lake Uluabat in Turkey was sampled during the summer of 2015. Phylogenetic analysis of the environmental mcyA sequences suggested Microcystis spp. were the major MC contributors. A combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS), liquid chromatography with UV detection and mass spectrometry (LC-UV-MS), and a novel liquid chromatography-high resolution mass spectrometry (LC-HRMS) method, together with thiol and periodate reactivity, revealed more than 36 MC variants in the lake samples and a strain of M. aeruginosa (AQUAMEB-24) isolated from Lake Uluabat. Only MCs containing arginine at position-4 were detected in the culture, while MC-LA, -LY, -LW and -LF were also detected in the lake samples, suggesting the presence of other MC producers in the lake. The previously unreported MCs MC-(H2)YR (dihydrotyrosine at position-2) (17), [epoxyAdda(5)]MC-LR, [DMAdda(5)]MC-RR (1) and [Mser(7)]MC-RR (8) were detected in the culture and/or field samples. This study is a good example of how commonly used targeted LC-MS methods can underestimate the diversity of MCs in freshwater lakes and cyanobacteria cultures and how untargeted LC-MS methods can be used to comprehensively assess MC diversity present in a new system. | en_US |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Y641] | en_US |
dc.description.sponsorship | This study was supported by a grant from The Scientific and Technological Research Council of Turkey (TUBITAK) (Project no: 114Y641). MY, MO and MB performed the field work. MO and MB prepared the samples for analyses. MB isolated the strain AQUAMEB24. MO performed ELISA and molecular analyses. ND performed cyanobacterial identifications and counts. AJF, COM, and DGB performed the LC-MS/MS analyses. MY planned the study, performed the phylogenetic analyses and wrote the paper with AJF, DGB and COM. The authors thank Cem Dirlik, Tolga Coskun and Gizem Seren Karatay for help during sampling and sample preparations, and K. Sivonen (Helsinki University, Helsinki, Finland) for providing Nostoc sp. strain 152. | en_US |
dc.identifier.doi | 10.1016/j.toxicon.2019.06.006 | en_US |
dc.identifier.endpage | 100 | en_US |
dc.identifier.issn | 0041-0101 | |
dc.identifier.pmid | 31181296 | en_US |
dc.identifier.scopusquality | Q3 | en_US |
dc.identifier.startpage | 87 | en_US |
dc.identifier.uri | http://doi.org/10.1016/j.toxicon.2019.06.006 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12885/596 | |
dc.identifier.volume | 167 | en_US |
dc.identifier.wos | WOS:000482248000012 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.institutionauthor | Yılmaz, Mete | |
dc.language.iso | en | en_US |
dc.publisher | Pergamon-Elsevier Science Ltd | en_US |
dc.relation.ispartof | Toxicon | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | [DMAdda(5)] MC-RR | en_US |
dc.subject | Lake uluabat | en_US |
dc.subject | MC-(H2)YR | en_US |
dc.subject | [Mser(7)]MC-RR | en_US |
dc.subject | Microcystin | en_US |
dc.subject | Untargeted LC-HAMS | en_US |
dc.title | Comprehensive multi-technique approach reveals the high diversity of microcystins in field collections and an associated isolate of Microcystis aeruginosa from a Turkish lake | en_US |
dc.type | Article | en_US |