Biochemical analysis of microbiotas obtained from healthy, prediabetic, type 2 diabetes, and obese individuals

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dc.contributor.authorDüzgün, Çağla
dc.contributor.authorDede, Süreyya
dc.contributor.authorKarakuş, Emine Karakuş
dc.contributor.authorAdas, Mine
dc.contributor.authorBilen, Ömer
dc.date.accessioned2026-02-08T15:08:05Z
dc.date.available2026-02-08T15:08:05Z
dc.date.issued2023
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractObjectives: In this study, we aimed to evaluate the intesti- nal and urinary microbiota diversity of obese, pre-diabetic, diabetic, and healthy subjects together with their food con- sumption frequency and investigate the effect on glucose metabolism. Methods: DNA was isolated from stool and urinary sam- ples of fifteen obese, fifteen prediabetics, fifteen type 2 diabetic, and fifteen lean participants by using the quantita- tive real-time polymerase chain reaction (qPCR) method. The amounts of Bifidobacterium, Bacteroides, and Firmicutes were measured and food consumption frequency was answered by all participants through a questionnaire. Results: The levels of Bifidobacterium in fecal microbiota were significantly higher in type 2 diabetic patients compared with lean (p=0.034), prediabetic (p=0.009), and obese par- ticipants (p=0.012). However, the levels of Bifidobacterium in urinary microbiota were decreased in obese, prediabetic, and type 2 diabetic subjects as controls (p=0.048; p=0.038; p=0.015 respectively). Additionally, Bacteroides/Firmicutes ratio decreased in type two diabetic patients compared with lean subjects and had a negative correlation with BMI in prediabetic subjects. Food consumption frequency illustrates that lean subjects have unhealthy eating habits. Conclusions: Urinary microbiota could be considered in the future context of a potential biomarker in the progress of type 2 diabetes and obesity.
dc.identifier.doi10.1515/tjb-2022-0110
dc.identifier.endpage65
dc.identifier.issn1303-829X
dc.identifier.issn1303-829X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85141161653
dc.identifier.scopusqualityN/A
dc.identifier.startpage58
dc.identifier.trdizinid1252499
dc.identifier.urihttps://doi.org/10.1515/tjb-2022-0110
dc.identifier.urihttps://hdl.handle.net/20.500.12885/4761
dc.identifier.volume48
dc.identifier.wosWOS:000871622400001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofTürk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260207
dc.subjectObesity
dc.subjectmicrobiota
dc.subjecttype 2 diabetes
dc.subjectprediabetes
dc.subjectreal-time qPCR
dc.titleBiochemical analysis of microbiotas obtained from healthy, prediabetic, type 2 diabetes, and obese individuals
dc.typeArticle

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