Surface coatings of contact lenses for cell sheet transplantation

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dc.authorid0000-0002-3064-6449
dc.authorid0000-0002-4904-7091
dc.authorid0000-0003-3255-4940
dc.authorid0000-0002-8584-1145
dc.contributor.authorCan, Gamze Dereli
dc.contributor.authorAtay, Merve Tunca
dc.contributor.authorEgil, Abdurrahim Can
dc.contributor.authorGuney, Eda
dc.contributor.authorShemshad, Sepideh
dc.contributor.authorTaner, Gokce
dc.contributor.authorInce, Gozde Ozaydin
dc.date.accessioned2026-02-08T15:14:45Z
dc.date.available2026-02-08T15:14:45Z
dc.date.issued2024
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractThe field of corneal cell sheet engineering has evolved rapidly in the last decade; however, challenges related to transplantation into target tissue still exist, necessitating a new approach to integrate cell sheet technology onto a contact lens surface. In this study, a novel surface-modified contact lens was developed for corneal regenerative medicine to ensure the easy and safe integration of cell sheets into the target tissue. The lens surface was coated with a thermo-responsive polymer using the initiated chemical vapor deposition technique, facilitating the separation of tissue-integrated cell sheets from the coated lens surface by simply lowering the temperature. BEAS-2B and L929 cells, along with human limbal epithelial cells, were cultured on a crosslinked poly(2-(dimethylamino)ethyl methacrylate)-coated lens, providing temperature sensitivity for 14 days. At the end of the incubation period, the cultured cells were harvested as intact monolayer cell sheets through a simple temperature reduction, without enzymatic treatment or additional surgical procedures. Rapid and complete delamination of the cells planted and incubated on the coated lens surface was achieved at room temperature. Therapeutic contact lenses modified with cell sheet technology allowed targeted delivery of cells to the affected area of the cornea. Schematics depict the initated chemical vapor deposition of a temperature responsive polymer coating on contact lenses, enabling the temperature controlled detachment of limbal epithelial cells for cell sheet transplantation. image
dc.description.sponsorshipLoreal Turkey Women in Science National Fellowship Program; Council of Higher Education (CoHE) PhD Scholar in Sustainable Agriculture subdivision [100/2000]; Council of Higher Education
dc.description.sponsorshipThis project was supported by the Loreal Turkey Women in Science National Fellowship Program (2019) in cooperation with the UNESCO Turkish National Commission. Merve Tunca Atay is a 100/2000 The Council of Higher Education (CoHE) PhD Scholar in Sustainable Agriculture subdivision. Sibel Cinar Asa is a 100/2000 The Council of Higher Education (CoHe) PhD Scholar in Molecular Pharmacology and Drug Investigations subdivision.
dc.identifier.doi10.1002/app.55458
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue23
dc.identifier.scopus2-s2.0-85189084442
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/app.55458
dc.identifier.urihttps://hdl.handle.net/20.500.12885/5407
dc.identifier.volume141
dc.identifier.wosWOS:001188904400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzWOS_KA_20260207
dc.subjectcell sheet technology
dc.subjectcontact lens
dc.subjectcorneal recovery
dc.subjectsurface modification
dc.subjecttemperature responsive coating
dc.subjecttransplantation
dc.titleSurface coatings of contact lenses for cell sheet transplantation
dc.typeArticle

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