Evaluation of the cytotoxic and genotoxic/antigenotoxic effects of resveratrol in human limbal explant cultures

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dc.authorid0000-0002-0290-1166
dc.authorid0000-0001-6814-0585
dc.authorid0000-0002-7642-0269
dc.contributor.authorCan, Gamze Dereli
dc.contributor.authorTaner, Gokce
dc.contributor.authorAydemir, Cigdem Inci
dc.date.accessioned2026-02-12T21:05:32Z
dc.date.available2026-02-12T21:05:32Z
dc.date.issued2023
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractPurpose Resveratrol (RSV) is a natural polyphenol phytoalexin compound and has long been considered to possess antioxidant and anti-inflammatory effects. In order to exploit the protective potential of RSV in anterior segment diseases, we investigated the possible cytotoxic, genotoxic/antigenotoxic effects of human limbal explant cultures to RSV and MMC or H2O2 alone and in combination. Methods A total of 18 limbal explant tissues obtained from three corneal donors were placed on the 12 well tissue culture polystyrene plates and cultured for 14 days. Cell growth from limbal explants was observed by inverted phase contrast microscopy. The cytotoxic effects of RSV was studied by neutral red uptake assay. For the assessment of the genotoxic and antigenotoxic effects, basic alkaline technique of comet assay was performed. Results It was found that the concentrations of RSV up to 100 mu M did not significantly affect the viability of outgrowth cells of limbal explant during 24 h exposure. When compared to negative control, all concentrations of RSV alone caused an increase in DNA strand breakage. Interestingly, 10 mu g/mL MMC alone caused similar tail intensity and tail moment values with RSV alone. On the other hand, RSV treatment in all doses seemed to decrease the DNA damage induced by either H2O2 or MMC. Conclusion RSV is an attractive natural compound for the purpose of oxidative stress reduction in ocular surface and can be utilized as a supplement to promote ocular surface regeneration in vivo.
dc.identifier.doi10.1007/s10792-022-02597-8
dc.identifier.endpage1985
dc.identifier.issn0165-5701
dc.identifier.issn1573-2630
dc.identifier.issue6
dc.identifier.pmid36445549
dc.identifier.scopus2-s2.0-85142924367
dc.identifier.scopusqualityQ2
dc.identifier.startpage1977
dc.identifier.urihttps://doi.org/10.1007/s10792-022-02597-8
dc.identifier.urihttps://hdl.handle.net/20.500.12885/7018
dc.identifier.volume43
dc.identifier.wosWOS:000890135000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofInternational Ophthalmology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260212
dc.subjectMitomycin C
dc.subjectResveratrol
dc.subjectOxidative stress
dc.subjectLimbal stem cell deficiency
dc.subjectLimbal explant culture
dc.subjectAlkaline comet assay
dc.titleEvaluation of the cytotoxic and genotoxic/antigenotoxic effects of resveratrol in human limbal explant cultures
dc.typeArticle

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