Guanine oxidation signal enhancement in single strand DNA with polyacrylonitrile/polyaniline (PAN/PAni) hybrid nanofibers

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dc.authorid0000-0001-7053-1075en_US
dc.contributor.authorCam, Ezginur
dc.contributor.authorTanik, Nilay Aladag
dc.contributor.authorÇerkez, İdris
dc.contributor.authorDemirkan, Elif
dc.contributor.authorAykut, Yakup
dc.date.accessioned2021-03-20T20:13:22Z
dc.date.available2021-03-20T20:13:22Z
dc.date.issued2018
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Polimer Malzeme Mühendisliği Bölümüen_US
dc.description.abstractPure polyacrylonitrile (PAN) and polyacrylonitrile/polyaniline (PAN/PAni) hybrid nanofibers (NFs) were produced via electrospinning and used to monitor guanine oxidation in single strand DNA (ssDNA) by electrochemical methods. Two different methodologies were conducted. First, pre-synthesized PAni was added into electrospinning PAN solution and electrospun into composite PAN/PAni nanofibrous structure on cylindrical pencil graphite (PGE) surface. In the second route, PAN NFs were electrospun on a PGE surfaces and polymerization of PAni was conducted on the surfaces of the as-spun PAN NFs. NFs were kept at -18 degrees C in a refrigerator for several days. ssDNA was immobilized on the prepared NFs and guanine oxidation signals were observed for each system. The results revealed that use of PAN NFs enhanced signal intensity from 0.92 mu A (PGE) to 1.04 mu A (PAN NFs). Addition of PAni to PAN increased signal intensity to 1.23 mu A. When the PAN NF surfaces were coated with PAni, signal enhancement continued to increase up to 4.19 mu A for fourth day and decreased again when PAni-coated NFs were kept at -18 degrees C in the refrigerator. Since the prepared system is fast and cheap, it is promising for application in DNA biosensor devices. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45567.en_US
dc.description.sponsorshipScientific Research Project Unit (BAP) of Uludag University [QUAP (MH) 2014/23]en_US
dc.description.sponsorshipThis study was supported by Scientific Research Project Unit (BAP) of Uludag University, under project number: QUAP (MH) 2014/23. Authors thank Uludag University. The study is Ezginur Cam's Master thesis at the Graduate School of Natural and Applied Sciences, Uludag University.en_US
dc.identifier.doi10.1002/app.45567en_US
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttp://doi.org/10.1002/app.45567
dc.identifier.urihttps://hdl.handle.net/20.500.12885/853
dc.identifier.volume135en_US
dc.identifier.wosWOS:000412517800002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇerkez, İdris
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Applied Polymer Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbiomedical applicationsen_US
dc.subjectelectrospinningen_US
dc.subjectmanufacturingen_US
dc.subjectmembranesen_US
dc.subjectnucleic acidsen_US
dc.titleGuanine oxidation signal enhancement in single strand DNA with polyacrylonitrile/polyaniline (PAN/PAni) hybrid nanofibersen_US
dc.typeArticleen_US

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