The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality

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dc.authorid0000-0001-8396-139X
dc.authorid0000-0001-8932-4535
dc.authorid0000-0003-4114-7044
dc.authorid0000-0002-4727-6990
dc.contributor.authorAllai, Larbi
dc.contributor.authorDruart, Xavier
dc.contributor.authorTerzioglu, Pinar
dc.contributor.authorLouanjli, Noureddine
dc.contributor.authorNasser, Boubker
dc.contributor.authorOzturk, Mehmet
dc.contributor.authorEl Amiri, Bouchra
dc.date.accessioned2026-02-12T21:05:33Z
dc.date.available2026-02-12T21:05:33Z
dc.date.issued2023
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractRecently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context, the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia fcus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5 degrees C. The antioxidant activity of ETHEX was studied using free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH center dot) assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES) and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaad rams with an artificial vagina. The ejaculates with more than 70% motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1-8% of ETHEX (37 degrees C; 0.8 x 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48, and 72 h. The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2% ETHEX in SM increased the sperm motility, viability, and membrane integrity and decreased the abnormality of spontaneous and catalyzed lipids peroxidation (p < 0.05) up to 72 h. In addition, semen diluted with 1 and 2% ETHEX decreased the level of DNA fragmentation compared to the control group (p < 0.05). In conclusion, the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on artificial insemination should be further studied.
dc.identifier.doi10.1155/2023/6248890
dc.identifier.issn2090-8113
dc.identifier.issn2042-0048
dc.identifier.pmid37063479
dc.identifier.scopus2-s2.0-85153197742
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1155/2023/6248890
dc.identifier.urihttps://hdl.handle.net/20.500.12885/7026
dc.identifier.volume2023
dc.identifier.wosWOS:000972658600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofVeterinary Medicine International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260212
dc.subjectLiquid Storage
dc.subjectPhenolic Composition
dc.subjectLipid-Peroxidation
dc.subjectSemen Quality
dc.subjectDna-Damage
dc.subjectBull Sperm
dc.subjectIn-Vitro
dc.subjectMotility
dc.subjectZinc
dc.subjectCryopreservation
dc.titleThe Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality
dc.typeArticle

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