Purification of Carbonic Anhydrase from Capoeta umbla (Heckel, 1843) Gills and Toxicological Effects of Some Metals on Enzyme Activity

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dc.authorid0000-0003-3667-6902en_US
dc.contributor.authorKirici, Muammer
dc.contributor.authorKirici, Mahinur
dc.contributor.authorBeydemir, Şükrü
dc.contributor.authorAtamanalp, Muhammed
dc.date.accessioned2021-03-20T20:14:39Z
dc.date.available2021-03-20T20:14:39Z
dc.date.issued2016
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Kimya Bölümüen_US
dc.description.abstractIn this study, in vitroeffects of some metal ions(Fe3+, Cd2+, Pb2+ and Ni2+)on cytoplasmic carbonic anhydrase(CA, EC 4.2.1.1) from Capoeta umbla gill was investigated. CA was purified from the gills of C. umbla for the first time. It was purified with the Sepharose-4B-L-Tyrosine Sulphanilamide affinity chromatography method. The overall purification was approx. 31.69 -fold with a yield of 53.33%, and a specific activity of 326.73 EU/mg proteins. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band corresponding to a molecular weight of approx. 29 kDa. The constants of the enzyme inhibitor complex (K-i) and 50% inhibitory values (IC50) for metal ions were determined by Lineweaver-Burk graphs and plotting activity % vs. [I], respectively. The Klconstants and IC50 values were 0.012 +/- 0.0135 and 0.136 mM for Fe3+, 0.019 +/- 0.0113 and 0.191 mM for Cd2+, 0.041 +/- 0.0075 and 0.289 mM for Pb2+, and 0,120 0.034 and 0.924 mM for Ni2+. It was determined that Fe3+, Cd2+ and Pb2+ inhibited the enzyme competitively while Ni2+ inhibited the enzyme noncompetitively. The potential inhibitor for C. umbla gill CA was found as Fe3+ from these results.en_US
dc.identifier.doi10.4194/1303-2712-v16_1_17en_US
dc.identifier.endpage175en_US
dc.identifier.issn1303-2712
dc.identifier.issn2149-181X
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage169en_US
dc.identifier.urihttp://doi.org/10.4194/1303-2712-v16_1_17
dc.identifier.urihttps://hdl.handle.net/20.500.12885/1086
dc.identifier.volume16en_US
dc.identifier.wosWOS:000378815400017en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorBeydemir, Şükrü
dc.language.isoenen_US
dc.publisherCentral Fisheries Research Insten_US
dc.relation.ispartofTurkish Journal Of Fisheries And Aquatic Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCapoeta umblaen_US
dc.subjectcarbonic anhydraseen_US
dc.subjectgillsen_US
dc.subjectmetal toxicityen_US
dc.titlePurification of Carbonic Anhydrase from Capoeta umbla (Heckel, 1843) Gills and Toxicological Effects of Some Metals on Enzyme Activityen_US
dc.typeArticleen_US

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