Observation of triple helix motif on electrospun collagen nanofibers and its effect on the physical and structural properties

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dc.authorid0000-0001-6389-2649en_US
dc.contributor.authorBuerck, Jochen
dc.contributor.authorAras, Onur
dc.contributor.authorBertinetti, Luca
dc.contributor.authorIlhan, Caner A.
dc.contributor.authorErmeydan, Mahmut Ali
dc.contributor.authorSchneider, Reinhard
dc.date.accessioned2021-03-20T20:13:23Z
dc.date.available2021-03-20T20:13:23Z
dc.date.issued2018
dc.departmentBTÜ, Orman Fakültesi, Orman Endüstri Mühendisliği Bölümüen_US
dc.description.abstractCollagen is a very popular natural biomaterial due to its high biocompatibility and bioactivity. Electro-spinning is currently the only technique that allows the fabrication of continuous fibers with diameters down to a few nanometers. In order to regenerate collagen in the forms of nanofibers, it is necessary to dissolve it in suitable solvents. The solvents and electrospinning process cause unfolding of collagen nanofibers. It is proposed that acidic solvents preserve better the natural structure of collagen fibers. In this paper, the structures of collagen nanofibers were examined by using circular dichroism (CD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, differential scanning calorimetry (DSC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods in order to test this hypothesis. The increase in PP-II fraction, representing the triple helix structure in collagen, that was observed in CD analysis of HAc derived collagen nanofibers, for the first time was successfully confirmed and illustrated by using SEM and TEM methods. Furthermore, CD revealed the mostly detrimental effect of stabilization conditions such as heat, vacuum and UV treatment on the secondary structure of the collagen nanofibers. (C) 2017 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112C025]en_US
dc.description.sponsorshipThis study is supported by TUBITAK's Co-Funded Brain Circulation Program-2236, (Project number 112C025). We acknowledge the Synchrotron Light Source ANKA for provision of instruments at their beamlines, and we would like to thank Bianca Posselt and Siegmar Roth (Institute for Biological Interfaces, IBG-2, KIT) for technical assistance during the SRCD measurements at the beamline UV-CD12.en_US
dc.identifier.doi10.1016/j.molstruc.2017.09.030en_US
dc.identifier.endpage80en_US
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage73en_US
dc.identifier.urihttp://doi.org/10.1016/j.molstruc.2017.09.030
dc.identifier.urihttps://hdl.handle.net/20.500.12885/855
dc.identifier.volume1151en_US
dc.identifier.wosWOS:000413886800009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorErmeydan, Mahmut Ali
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Molecular Structureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrospinningen_US
dc.subjectCollagenen_US
dc.subjectSEMen_US
dc.subjectTEMen_US
dc.subject3D native structureen_US
dc.titleObservation of triple helix motif on electrospun collagen nanofibers and its effect on the physical and structural propertiesen_US
dc.typeArticleen_US

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