Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2

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dc.authorscopusid57275159900en_US
dc.contributor.authorDogan, Kubra
dc.contributor.authorErol, Ebru
dc.contributor.authorDidem Orhan, Muge
dc.contributor.authorDegirmenci, Zehra
dc.contributor.authorYaşa, Belkıs
dc.date.accessioned2022-04-11T13:32:46Z
dc.date.available2022-04-11T13:32:46Z
dc.date.issued2021en_US
dc.departmentBTÜ, Orman Fakültesi, Orman Endüstri Mühendisliği Bölümüen_US
dc.description.abstractIntroduction: Numerous efforts in natural product drug development are reported for the treatment of Coronavirus. Based on the literature, among these natural plants Artemisia annua L. shows some promise for the treatment of SARS-CoV-2. Objective: The main objective of our study was to determine artemisinin content by liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS), to investigate the in vitro biological activity of artemisinin from the A. annua plants grown in Turkey with various extracted methods, to elaborate in silico activity against SARS-CoV-2 using molecular modelling. Methodology: Twenty-one different extractions were applied. Direct and sequential extractions studies were compared with ultrasonic assisted maceration, Soxhlet, and ultra-rapid determined artemisinin active molecules by LC-ESI-MS/MS methods. The inhibition of spike protein and main protease (3CL) enzyme activity of SARS-CoV-2 virus was assessed by time resolved fluorescence energy transfer (TR-FRET) assay. Results: Artemisinin content in the range 0.062–0.066%. Artemisinin showed significant inhibition of 3CL protease activity but not Spike/ACE-2 binding. The 50% effective concentration (EC50) of artemisinin against SARS-CoV-2 Spike pseudovirus was found greater than 50 μM (EC45) in HEK293T cell line whereas the cell viability was 94% of the control (P < 0.01). The immunosuppressive effects of artemisinin on TNF-α production on both pseudovirus and lipopolysaccharide (LPS)-induced THP-1 cells were found significant in a dose dependent manner. Conclusion: Further studies of these extracts for COVID-19 treatment will shed light to seek alternative treatment options. Moreover, these natural extracts can be used as an additional treatment option with medicines, as well as prophylactic use can be very beneficial for patientsen_US
dc.identifier.doihttps://doi.org/10.1002/pca.3088en_US
dc.identifier.endpage319en_US
dc.identifier.issn09580344
dc.identifier.issue2en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage303en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12885/1894
dc.identifier.volume33en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYaşa, Belkıs
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Ltden_US
dc.relation.ispartofPhytochemical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak18AG003
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectantiviral activityen_US
dc.subjectArtemisia annuaen_US
dc.subjectSARS-CoV-2en_US
dc.subjectLC-ESI-MS/MSen_US
dc.subjectnatural products for COVID-19en_US
dc.titleInstant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2en_US
dc.typeArticleen_US

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