Acacia catechu ethanolic bark extract induces apoptosis in human oral squamous carcinoma cells

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dc.contributor.authorLakshmi, Thangavelu
dc.contributor.authorEzhilarasan, Devaraj
dc.contributor.authorVijayaragavan, Rajagopal
dc.contributor.authorBhullar, Sukhwinder Kaur
dc.contributor.authorRajendran, Ramasamy
dc.date.accessioned2021-03-20T20:13:41Z
dc.date.available2021-03-20T20:13:41Z
dc.date.issued2017
dc.departmentBTÜ, Mühendislik ve Doğa Bilimleri Fakültesi, Makine Mühendisliği Bölümüen_US
dc.description.abstractOral cancer is in approximately 30% of all cencers in India. This study was conducted to evaluate the cytotoxic activity of ethanolic extract of Acacia catechu bark (ACB) against human squamous cell carcinoma cell line-25 (SCC-25). Cytotoxic effect of ACB extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide assay. A. catechu extract was treated SCC-25 cells with 50 mu g/mL for 24h. Apoptosis markers such as capases-8 and 9, bcl-2, bax, and cytochrome c(Cyt-c) were done by RT-PCR. Morphological changes of ACB treated cells were evaluated using acridine orange/ethidium bromide (AO/EB) dual staining. Nuclear morphology and DNA fragmentation were evaluated using propidum iodide (PI) straining. Further, cell cycle analysis was performed using flow cytometry.A. catechu treatment caused cytotoxicity in SCC-25 cells with an IC50 of 52.09 mu g/mL. Apoptotic marker gene expressions were significantly increased on ACB tratment. Staining with AO/EB and PI shows membrane biebbing and nuclear membrane disortion, respectively, and it confirms the apoptosis induction in SCC-25 cells. These results suggest that ACB extract can be used as a modulating agent in oral squamous cell carcinoma.en_US
dc.identifier.doi10.4103/japtr.JAPTR_73_17en_US
dc.identifier.endpage149en_US
dc.identifier.issn2231-4040
dc.identifier.issn0976-2094
dc.identifier.issue4en_US
dc.identifier.pmid29184846en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage143en_US
dc.identifier.urihttp://doi.org/10.4103/japtr.JAPTR_73_17
dc.identifier.urihttps://hdl.handle.net/20.500.12885/920
dc.identifier.volume8en_US
dc.identifier.wosWOS:000413761900007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBhullar, Sukhwinder Kaur
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofJournal Of Advanced Pharmaceutical Technology & Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCaspasesen_US
dc.subjectcytotoxicityen_US
dc.subjectnuclear membraneen_US
dc.subjectsquamous cell carcinoma cell line-25 cellsen_US
dc.titleAcacia catechu ethanolic bark extract induces apoptosis in human oral squamous carcinoma cellsen_US
dc.typeArticleen_US

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