Optimization of culture conditions for the production and activity of recombinant xylanase from microalgal platform

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dc.authorid0000-0001-9836-3181
dc.authorid0000-0003-4106-4548
dc.authorid0000-0003-1880-4261
dc.contributor.authorYildirim, Arzu
dc.contributor.authorIlhan-Ayisigi, Esra
dc.contributor.authorDuzel, Ahmet
dc.contributor.authorMayfield, Stephen Patrick
dc.contributor.authorSargin, Sait
dc.date.accessioned2026-02-12T21:05:35Z
dc.date.available2026-02-12T21:05:35Z
dc.date.issued2023
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractXylanases are enzymes responsible for the hydrolysis of the heteropolymer xylan. They have wide applications ranging from the bakery, animal husbandry, and textile to pulp and paper industry and biofuel productions. Recombinant xylanase production has been previously reported from different hosts such as bacteria and yeast. Microalgae offer a safe and cost-effective photosynthetic platform for producing recombinant proteins, including therapeutics and industrial enzymes. In this study, we optimized the production of recombinant xylanase expressed and secreted from the green alga, Chlamydomonas reinhardtii. The growth of the culture was optimized using response surface methodology (RSM) based on central composite design (CCD), with two numeric (culture incubation time and agitation rate) and one categoric (light intensity) factors. The optimum biomass concen-tration was obtained as 0.71 mg/mL from the CCD values. In addition, bubble column photobioreactors were set and compared for the culture growth, the protein concentration, and the enzyme activity under different light intensities and air flows. Increasing the aeration rate from 1 vvm to 2 vvm resulted in improved enzyme activity from 5330.5 U/g to 6277.7 U/g under 3500 lux illumination on the 3rd day of the culture. This study may lead to the further large-scale production of xylanase with high enzyme activity and reveal the advantage of the microalgae as a sustainable platform.
dc.identifier.doi10.1016/j.bej.2023.108967
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.scopus2-s2.0-85160277768
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.bej.2023.108967
dc.identifier.urihttps://hdl.handle.net/20.500.12885/7041
dc.identifier.volume197
dc.identifier.wosWOS:001007103600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260212
dc.subjectMicroalgae
dc.subjectXylanase
dc.subjectRecombinant enzyme
dc.subjectResponse surface methodology
dc.subjectPhotobioreactor
dc.titleOptimization of culture conditions for the production and activity of recombinant xylanase from microalgal platform
dc.typeArticle

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