Inhibition and purification of acetylcholinesterases from adults and nymphs of Ricania simulans (Walker, 1851) (Hemiptera: Ricaniidae)

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dc.authorid0000-0001-9591-5411
dc.contributor.authorDincer, Barbaros
dc.contributor.authorKizil, Demet
dc.date.accessioned2026-02-12T21:04:59Z
dc.date.available2026-02-12T21:04:59Z
dc.date.issued2022
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractRicania simulans (Walker, 1851) (Hemiptera: Ricaniidae) has been causing damage in agricultural areas along the eastern Black Sea coastline since 2006. As there are not enough studies on managing this pest, it continues to pose a threat. One of the significant control tactics of insecticides includes the inhibition of acetylcholinesterase. Therefore the present study is aimed to study an alternative way to combat R. simulans through inhibition of acetylcholinesterase (ACHE). The ACHE was purified from adults and nymphs of R. simulans one by one using one-step edrophonium-Sepharose 6B affinity chromatography, and some kinetic properties were studied. The ACHEs were purified 251.6-fold with a yield of 34.2% for adults and 65.5-fold with a yield of 2.2% for nymphs. The K-m, V-max, and k(cat) values of R. simulans ACHE for acetylthiocholine iodide (ATC) hydrolysis were determined to be 0.04 +/- 0.01 mM, 2,000.0 +/- 250 EU/mg protein and 104.5 +/- 13 min(-1) for adults and 0.02 +/- 0.01 mM, 500.0 +/- 52 EU/mg protein and 30.3 +/- 6.4 min(-1) for nymphs, respectively. In inhibition studies of R. simulans ACHEs with edrophonium chloride, tacrine and olive leaf extract with water, the IC50 values of the inhibitors were calculated. Although all inhibitors inhibited the ACHEs, they inhibited the nymphs' ACHEs more effectively. In particular, the fact that the olive leaf water extract is effective in the ACHEs inhibition of these two stages is important for it to be an environmentally friendly pesticide that can be used in the fight against this pest.
dc.description.sponsorshipResearch Fund of Recep Tayyip Erdogan University [:1184]
dc.description.sponsorshipThis work was financially supported by the Research Fund of Recep Tayyip Erdogan University (Project ID:1184).
dc.identifier.doi10.1007/s42690-022-00835-1
dc.identifier.endpage3372
dc.identifier.issn1742-7584
dc.identifier.issn1742-7592
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85132554088
dc.identifier.scopusqualityQ2
dc.identifier.startpage3363
dc.identifier.urihttps://doi.org/10.1007/s42690-022-00835-1
dc.identifier.urihttps://hdl.handle.net/20.500.12885/6753
dc.identifier.volume42
dc.identifier.wosWOS:000814958600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofInternational Journal of Tropical Insect Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260212
dc.subjectAcetylcholinesterase
dc.subjectRicania simulans
dc.subjectPurification
dc.subjectInhibition
dc.titleInhibition and purification of acetylcholinesterases from adults and nymphs of Ricania simulans (Walker, 1851) (Hemiptera: Ricaniidae)
dc.typeArticle

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