ASSESSMENT OF CELL COUNTING METHOD BASED ON IMAGE PROCESSING FOR A MICROALGA CULTURE

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dc.contributor.authorDökümcüoğlu, Vesile Esra
dc.contributor.authorYılmaz, Mete
dc.date.accessioned2026-02-08T15:04:59Z
dc.date.available2026-02-08T15:04:59Z
dc.date.issued2020
dc.departmentBursa Teknik Üniversitesi
dc.description.abstractRoutine monitoring of microalgal growth requires the use of one of several methods such as cell counting under the microscope and measuring optical density (OD) with a spectrophotometer. Each of these methods has their advantages and disadvantages. For example, counting cells under the microscope can be time consuming, but it provides the best estimate of cell growth. Measuring OD is much quicker, however it doesn’t provide any information on cell numbers and debris in culture can interfere with OD measurements. Therefore, this study aimed to demonstrate the usefulness of an image processing approach for counting cells in a microalga culture. Results showed that highest correlations were observed between Utermöhl cell counts and OD measurements (r=0.99), ImageJ cell counts and OD measurements (r=0.99) and between Utermöhl and ImageJ cell counts (r=0.99). In the regression analysis, highest R2 values were obtained for Utermöhl vs OD (R2=0.99) and ImageJ vs OD (R2=0.99). Counting algal cells with ImageJ allows the analyst to complete the procedure 4 times faster than with manual Utermöhl procedure.
dc.identifier.endpage81
dc.identifier.issn2618-6551
dc.identifier.issue2
dc.identifier.startpage75
dc.identifier.urihttps://hdl.handle.net/20.500.12885/4352
dc.identifier.volume3
dc.language.isoen
dc.publisherMersin University
dc.relation.ispartofMediterranean Fisheries and Aquaculture Research
dc.relation.ispartofMediterranean Fisheries and Aquaculture Research
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20260207
dc.subjectHydrobiology
dc.subjectHidrobiyoloji
dc.titleASSESSMENT OF CELL COUNTING METHOD BASED ON IMAGE PROCESSING FOR A MICROALGA CULTURE
dc.typeArticle

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