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Öğe Characterization of prodigiosin pigment by Serratia marcescens and the evaluation of its bioactivities(Pergamon-Elsevier Science Ltd, 2022) Koyun, Merve Tunca; Sirin, Seda; Aslim, Belma; Taner, Gokce; Dolanbay, Serap NigdeliogluThe aim of the present study is to discover a bacterial pigment providing protection and prevention of neurological damage and cancer development, which can have a role as a non-synthetic food additive in the food industry as well as an active drug ingredient of anticancer drugs and pharmaceuticals for neural injury. Within this scope, Serratia marcescens MB703 strain was used to produce prodigiosin. Characterization of the prodigiosin was carried out using UV-VIS, and FT-IR. In addition, its inhibitory action on AChE and antioxidant activities were determined. The cytotoxic, genotoxic and antigenotoxic activities of the prodigiosin as well as its anti proliferative activities were detected. It was determined that the maximum production of the prodigiosin (72 mg/L). The prodigiosin was found to cause no significant difference in its inhibitory effect on AChE. The prodigiosin was found effective on all antioxidant parameters tested. The IC50 values of the prodigiosin on SK-MEL30 and HT-29 cells were calculated as 70 and 47 mu M, respectively. This IC50 values of the prodigiosin showed no cytotoxic effect on L929 cells. Prodigiosin did not have genotoxic effect alone and also seem to decrease DNA damage induced by H2O2 in L929 cells. The findings of in vitro experimental studies suggest that using the prodigiosin pigment as a drug candidate for cancer and neurodegenerative disease therapy is both effective and safe.Öğe Chronic Occupational Exposure to Chemical Mixtures Induces Genomic Instability in Paint Workers(Mdpi, 2025) Iritas, Servet Birgin; Bacanli, Merve Gudul; Taner, Gokce; Turksoy, Vugar Ali; Tutkun, Lutfiye; Yilmaz, Omer Hinc; Basaran, Ayse NursenThis study's objective was to evaluate genotoxic effects on automotive paint workers who are exposed to a complex mixture of VOCs, heavy metals, and solvents. Biological samples, including blood, urine, and buccal epithelial cells, were collected from 80 exposed workers and 80 demographically matched control subjects. DNA damage was assessed using the alkaline COMET assay in lymphocytes and whole blood. The Buccal Micronucleus Cytome (BMCyt) assay was also employed to identify cytogenetic abnormalities. Additionally, trichloroacetic acid (TCA), hippuric acid (HA), phenol, and lead (Pb) levels were measured as biomarkers of exposure. A significant increase in DNA damage was observed in the lymphocytes and whole blood of exposed workers (p < 0.05) BMCyt analysis also revealed higher frequencies of micronuclei (MN), binucleated cells, condensed chromatin (CC), and karyorrhectic (KHC) and pyknotic cells (PYC) in buccal cells (p < 0.05). Elevated levels of urinary HA, phenol, TCA, and blood lead indicated systemic chemical exposure. DNA damage positively correlated with these biomarkers, supporting a strong link between chronic occupational exposure and genotoxicity. The findings from this study highlight the critical importance of implementing effective safety measures and consistent biomonitoring for paint workers to prevent adverse health effects.Öğe Cytotoxic and genotoxic effects of leaking chemicals from serum infusion sets: an in-vitro study(Oxford Univ Press, 2023) Ozlu, Aysegul; Taner, GokceSafety concerns about medical devices playing important role in health sciences and bioengineering research are rising day by day. Although there are specific standards regarding disposable medical materials, the information is updating with the toxicological studies. In this study, cytotoxic/genotoxic effects of chemicals leaking from serum infusion sets that have an important place in the clinic were investigated. Media containing leakage chemicals were prepared from equal samples taken from the plastic line sections of 13 different brands of serum infusion sets containing phthalates and the effects on the cultured cells were compared with the untreated control groups. To obtain leaking chemicals, extracting period was selected as 72 h, a routine set-change time in the clinic as indicated in various publications. Neutral red uptake and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests were performed in L929 cells to determine cytotoxicity, and cytokinesis blocked micronucleus technique was performed in lymphocytes to determine genotoxicity. Cytotoxic and genotoxic damage levels were compared by evaluating cell-viability rates relative to control, micronucleus frequency, and nuclear division index values. The results showed that all sets caused a decrease in cell viability revealing the effects both on lysosomal and mitochondrial activity and increase in micronucleus frequencies in general. The number of similar studies is extremely limited, and in this study in addition to the short-term effects of using the serum infusion sets, the information about the sample tests to determine the biosecurity of disposable medical materials is given.Öğe Encapsulated black garlic: Comparison with black garlic extract in terms of physicochemical properties, biological activities, production efficiency and storage stability(Elsevier, 2022) Cinar, Aycan; Altuntas, Seda; Demircan, Huseyin; Dundar, Ayse Neslihan; Taner, Gokce; Oral, Rasim AlperIn this paper, the convenience of encapsulation technique was investigated in terms of characterization of capsule, changing the volatile compounds and preservation biological activity throughout six months for black garlic (BG) by comparison with the aqueous extract which prepared from BG (stored at 4 C) each analysis period. Besides, the genotoxic and antigenotoxic aspects of aqueous black garlic extract (BGE) and black garlic capsule (BGC) developed as a novel promising product were also researched. The S-allyl-L-cysteine (SAC) con-tent, which is mostly known to be responsible for biological activity, was preserved in BGC during six months. The variety of volatile compound of BGC (2 compounds) was significantly reduced compared to BG (17 com-pounds) and BGE (7 compounds), which indicates that the extraction and encapsulation traps the undesirable odor. A significant decrease was observed for total phenolic content and antioxidant capacity (performed with DPPH and CUPric Reducing Antioxidant Capacity (CUPRAC)) of BGC with storage like BGE. In contrast, the values obtained with the ABTS method remained constant with storage. The antimicrobial activity (carried out on five Gram-positive bacteria, three Gram-negative bacteria and three yeasts) of BGC was preserved better than BGE. The treatment concentrations (50, 100, 200 mu g/mL) of both BGE and BGC did not have a genotoxic effect and moreover they have antigenotoxic effects against mitomycin C on human lymphocyte cells. All these results implied that BGC can be good a candidate to increase the consumption of BG providing health benefits and functional supplement for food.Öğe Enhanced antibacterial and genoprotective properties of nanoliposomal Satureja hortensis L. essential oil(Taylor & Francis Ltd, 2025) Cagal, Munevver Muge; Taner, Gokce; Kalayci, Sadik; Duman, GulengulNanoliposomes are drug delivery systems that improve bioavailability by encapsulating therapeutic agents. The main objective of this study was to investigate the effects of nanoliposomal (NL) formulation on enhancing the bioavailability of essential oil. The essential oil of Satureja hortensis (SHO) was encapsulated in nanoliposomes (SHNLs). Physicochemical characterizations of NL formulations (size, charge, polydispersity index [PDI]) were evaluated by dynamic light scattering technique. The nanoliposome encapsulation efficiency (EE) was calculated as 89.90%. The prepared bionanosystems demonstrated significant antibacterial activities against Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 15442, and Staphylococcus aureus ATCC as determined by the agar diffusion method and microdilution tests. Minimum inhibitory concentration (MIC) values for SHNLs were found to be 5.187 mu g/mu L for E. coli and 2.59 mu g/mu L for both P. aeruginosa and S. aureus. Importantly, despite the lower substance content, both SHNLs and SHO exhibited comparable antibacterial activity against all tested strains. Furthermore, in order to determine the toxicity profile and possible effects on DNA damage or repair both the genotoxic and antigenotoxic effects of SHNLs were assessed using the cytokinesis-blocked micronucleus (CBMN) method in human lymphocyte cultures. The experimental data collectively indicate that the NL formulation of the S. hortensis essential oil enhances antibacterial activities and provides genoprotective effects against DNA damage. This highlights the significance of liposomal formulations of antioxidants in augmenting their biological activity. The results indicate that SHNLs can be a safe antibacterial agent for the pharmaceutical industry.Öğe Evaluation of the cytotoxic and genotoxic/antigenotoxic effects of resveratrol in human limbal explant cultures(Springer, 2023) Can, Gamze Dereli; Taner, Gokce; Aydemir, Cigdem InciPurpose Resveratrol (RSV) is a natural polyphenol phytoalexin compound and has long been considered to possess antioxidant and anti-inflammatory effects. In order to exploit the protective potential of RSV in anterior segment diseases, we investigated the possible cytotoxic, genotoxic/antigenotoxic effects of human limbal explant cultures to RSV and MMC or H2O2 alone and in combination. Methods A total of 18 limbal explant tissues obtained from three corneal donors were placed on the 12 well tissue culture polystyrene plates and cultured for 14 days. Cell growth from limbal explants was observed by inverted phase contrast microscopy. The cytotoxic effects of RSV was studied by neutral red uptake assay. For the assessment of the genotoxic and antigenotoxic effects, basic alkaline technique of comet assay was performed. Results It was found that the concentrations of RSV up to 100 mu M did not significantly affect the viability of outgrowth cells of limbal explant during 24 h exposure. When compared to negative control, all concentrations of RSV alone caused an increase in DNA strand breakage. Interestingly, 10 mu g/mL MMC alone caused similar tail intensity and tail moment values with RSV alone. On the other hand, RSV treatment in all doses seemed to decrease the DNA damage induced by either H2O2 or MMC. Conclusion RSV is an attractive natural compound for the purpose of oxidative stress reduction in ocular surface and can be utilized as a supplement to promote ocular surface regeneration in vivo.Öğe Nanoliposomal system for augmented antibacterial and antiproliferative efficacy of Melissa officinalis L. extract(Oxford Univ Press, 2024) Nizam, Nagihan; Taner, Gokce; Cagal, Munevver MugeObjective This study focused on the nanoliposomal encapsulation of bioactive compounds extracted from Melissa officinalis L. (ME) using ethanol as a strategy to improve the antibacterial activity, anticytotoxic, and antiproliferative properties.Methods Nanoliposomes loaded with ME (MEL) were characterized for total phenolic content, particle size, polydispersity, and encapsulation efficiency. The minimum inhibitory concentration (MIC) values for MEL and ME were determined to evaluate antibacterial activity. To examine the toxicity profiles of ME and MEL, tests were conducted on the A549 and BEAS-2B cell lines using the MTT assay. Furthermore, an in vitro sctrach assay was conducted to evaluate the antiproliferative effects of ME and MEL on A549 cells.Results Nanoliposomes presented entrapment efficiency higher than 80%, nanometric particle size, and narrow polydispersity. The MIC values for MEL and ME were observed as 93.75 mu g/mu L against E. coli. MIC values for MEL and ME were achieved as 4.68 mu g/mu L and 9.375 mu g/mL against S. aureus, respectively. The IC50 values for ME were determined to be 1.13 mg/mL and 0.806 mg/mL, while the IC50 values for MEL were found to be 3.5 mg/mL and 0.868 mg/mL on A549 and BEAS-2B cell lines, respectively. Additionally, The MEL showed an antiproliferative effect against A549 cells at 500 mu g/mL concentration.Conclusion All experimental findings unequivocally demonstrate that the novel nanoliposomal system has effectively augmented the antibacterial activities and antiproliferative effects of ME. The initial findings indicate that nanoliposomes could effectively serve as carriers for ME in pharmaceutical applications.Öğe Preparation and characterization of Pickering foams by mechanical frothing and emulsion templating(Budapest Univ Technol & Econ, 2024) Parin, Fatma Nur; Dinc, Hatice; Parin, Ugur; Kildali, Elife; Taner, GokceIn this study, Pickering foamed emulsions have been prepared using beta-cyclodextrin (beta-CD), and d-limonene as a surfactant and an oil phase, respectively. The incorporation of beta-CD/d-limonene inclusion complexes (ICs) in specific proportions (1:1, 1:3, and 1:5) to water phase, which is a polymer matrix composed of a mixture of polyvinyl alcohol (PVA) and psyllium husk (PSH) by mechanical frothing at high speed, and air bubbles have been formed in oil in water (o/w) emulsions. Ecofriendly bio-based foams have been developed in this method. Scanning Electron Microscope (SEM) analysis showed PVA/PSH Pickering foams usually open porous morphologies and the addition of d-limonene increases the amount of porosity from 43 to 49%. Although the resulting foams indicated similar thermal degradation profile, the presence of d-limonene in foams increased thermal stability. The surfaces of foams have a hydrophilic property with contact angles values lower than 80 degrees. The tensile strength of foams decreased from 170 to 100 kPa due to the increased porosity. All foams indicated antibacterial activity to Staphylococcus aureus (S. aureus) with 9-12 mm zone inhibition. The incorporation of d-limonene into foams surprisingly decreased the cell viability. In brief, our findings show that the Pickering foams can be beneficial for wound healing applications.Öğe Surface coatings of contact lenses for cell sheet transplantation(Wiley, 2024) Can, Gamze Dereli; Atay, Merve Tunca; Egil, Abdurrahim Can; Guney, Eda; Shemshad, Sepideh; Taner, Gokce; Ince, Gozde OzaydinThe field of corneal cell sheet engineering has evolved rapidly in the last decade; however, challenges related to transplantation into target tissue still exist, necessitating a new approach to integrate cell sheet technology onto a contact lens surface. In this study, a novel surface-modified contact lens was developed for corneal regenerative medicine to ensure the easy and safe integration of cell sheets into the target tissue. The lens surface was coated with a thermo-responsive polymer using the initiated chemical vapor deposition technique, facilitating the separation of tissue-integrated cell sheets from the coated lens surface by simply lowering the temperature. BEAS-2B and L929 cells, along with human limbal epithelial cells, were cultured on a crosslinked poly(2-(dimethylamino)ethyl methacrylate)-coated lens, providing temperature sensitivity for 14 days. At the end of the incubation period, the cultured cells were harvested as intact monolayer cell sheets through a simple temperature reduction, without enzymatic treatment or additional surgical procedures. Rapid and complete delamination of the cells planted and incubated on the coated lens surface was achieved at room temperature. Therapeutic contact lenses modified with cell sheet technology allowed targeted delivery of cells to the affected area of the cornea. Schematics depict the initated chemical vapor deposition of a temperature responsive polymer coating on contact lenses, enabling the temperature controlled detachment of limbal epithelial cells for cell sheet transplantation. imageÖğe The role of microencapsulation in maintaining biological activity of royal jelly: comparison with biological activity and bioaccessibility of microencapsulated, fresh and lyophilized forms during storage(Wiley, 2022) Dundar, Ayse Neslihan; Cinar, Aycan; Altuntas, Seda; Ulubayram, Neslihan; Taner, Gokce; Dagdelen, Adnan Fatih; Oral, Rasim AlperBACKGROUND Royal jelly (RJ) is a unique beehive product and has been recommended for human health since ancient times because of its antioxidant, antimicrobial, antiproliferative, neuroprotective, anti-lipidemic and anti-aging features. However, the biggest obstacle in the use of RJ is the need for cold storage and the instability of bioactive components over time. In the present study, 10-hydroxy-2-decenoic acid (10-HDA) content, as well as antioxidant [using 1,1-diphenyl-2-picrylhydrazy and 2,2 '-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) methods] and antimicrobial activity (five Gram-positive, five Gram-negative and three yeasts), were comparatively evaluated for three RJ forms, two of which can be stored at 24 +/- 1 degrees C during storage. RESULTS Microencapsulated royal jelly (MRJ) stored at room temperature succeeded in preserving its 10-HDA content, a major bioactive compound, during the 6 months, with respect to lyophilized royal jelly (LRJ) and fresh RJ stored at 4 degrees C. The initial 10-HDA contents of RJ, LRJ and MRJ were determined as 1.90%, 5.26% and 2.75%, respectively. Moreover, the total phenolic content, antioxidant capacity and antimicrobial activity mostly remained constant throughout the storage period (P >= 0.05). Gram-positive strains were generally more sensitive than Gram-negative strains. In the present study, the in vitro simulated digestion analysis showed that MRJ can tolerate the digestion process. CONCLUSION Overall, the encapsulation process was considered as one preservative technique for RJ. The microencapsulation of RJ as shown in the results of the present study are encouraging in terms of enabling the local beekeeping sector to achieve ease of production and increased product diversity. MRJ shows promise as a commercial product with a high export value for producers. (c) 2022 Society of Chemical Industry.
