Yazar "Oskay, F." seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Ceratocystis platani is killing plane trees in Istanbul (Turkey)(Wiley, 2018) Lehtijaervi, Asko Tapio; Oskay, F.; Lehtijarvi, H. T. Dogmus; Kaya, A. G. Aday; Pecori, F.; Santini, A.Ceratocystis platani was detected at several locations in the European side of Istanbul, causing severe dieback and mortality, mainly on Platanusxacerifolia imported from Italy approximately 160years ago, but also on Platanus orientalis. In the work reported here, the causal agent of the dieback and mortality was identified based on morphological characteristics in culture and internal transcribed spacer (ITS) sequencing, confirming the presence of C.platani in Turkey. All analysed C.platani isolates from Istanbul shared the same ITS sequence with European C.platani strains available in the NCBI, but differed from other species previously ascribed to the Ceratocystis fimbriata species complex. Pathogenicity of C.platani was proven by inoculation onto 25-cm-long and 4-9mm diam. P.orientalis twigs and the causal agent reisolated from symptomatic tissues. In a survey of 976 Platanus in streets and parks of Istanbul, 26.5% of trees showed symptoms of C.platani infections, whilst 5.6% were dead. The proportion of symptomatic trees was higher where management interventions, such as topping or pruning and felling infected trees had been carried out.Öğe Heterobasidion Infection in Abies nordmanniana ssp. bornmülleriana Stands in Kastamonu Province(2012) Lehtijaervi, Asko Tapio; Lehtijarvi, H. T. Doğmuş; Ünal, S.; Karadeniz, M.; Aday, A. G. Kaya; Oskay, F.In this study, the proportion of Abies bornmülleriana trees infected by Heterobasidion spp. was investigated in Kastamonu Province in different types of stands. One 1 cm-thick disc was taken from each of 100 freshly cut trees, washed under a running tap, placed into plastic bags and incubated in growth chamber at 24 C° for 7 days. The area occupied by the conidial stage of Heterobasidion annosum s.l. was determined under a stereomicroscope using transparent film placed onto the upper surface of each disc. During this investigation conidia of Heterobasidion annosum s.l. were taken with a needle and placed onto agar plates. The obtained isolates were identified with pairing tests and DNA-based methods. All 36 isolates responded as Heterobasidion abietinum to the tester isolates. Identification based on PCR amplification with MJF – MJR and KJF-KFR primers gave the same result. 34% (34 out of 100) of the discs taken from the fir forests were found to be infected with H. abietinum. The characteristics of the colonized patches on the discs indicated that the H. abietinum colonies originated from stem infections of the trees. The proportion of the disc area covered by the conidial stage of the fungus was 80% in one of the discs, 6-10% in 6 discs, 1-5% in 18 discs, and 0-1 % in 11 discs. The conidiophores were observed in heartwood in only five samples.Öğe Impacts of inoculation with Herpotrichia pinetorum, Gremmenia infestans and Gremmeniella abietina on Pinus nigra subsp pallasiana and Cedrus libani seedlings in the field(Wiley, 2016) Dogmus-Lehtijarvi, H. T.; Lehtijaervi, Asko Tapio; Woodward, S.; Oskay, F.Herpotrichia pinetorum, Gremmenia infestans and Gremmeniella abietina were inoculated onto 2-year-old Anatolian black pine (Pinus nigra subsp. pallasiana) and Taurus cedar (Cedrus libani) seedlings planted in a high mountain forest (1800m a.s.l) in south-western Turkey, to determine the effects of these fungi during winter. In June, 8months after inoculation, 39.9% of experimental plants were dead and 20.4% of the surviving plants failed to flush. Gremmeniella abietina and H.pinetorum caused the most fatalities. Prevention of new shoot formation on surviving plants, however, was mainly an effect of G.abietina infections, although many surviving plants inoculated with G.infestans or H.pinetorum also failed to flush. All three pathogens had the potential to severely damage young plants of P.nigra subsp. pallasiana and C.libani growing at high elevations near to forests with heavy inoculum loads. The implications of this finding for P.nigra afforestations at high altitudes in Turkey are discussed. This study is also the first to report that G.infestans can infect and cause disease on young C.libani plants.Öğe Preliminary Results of Wood Endophytes of Abies cilicica in Yenişarbademli of Isparta Province(2012) Lehtijaervi, Asko Tapio; Lehtijarvi, H. T. Doğmuş; Aday, A. G. Kaya; Oskay, F.Fungal colonisation originating from endophytic thalli in wood of healthy Taurus fir (Abies cilicica) was studied. Bore cores were taken from living trees during field surveys. The bore cores were cut into 0.5 cm long fragments, washed with 70% (v/v) ethanol, flamed and placed into petri dishes containing malt extract, agar and streptomycin. The plates were incubated at 25°C for 4 weeks. The outgrowing mycelia were pure-cultured and identified based on amplification and sequencing of the internal transcribed spacer (ITS) region of their nuclear ribosomal DNA. Totally 12 different fungal species were isolated from Abies cilicica wood cores. Five of them belong to Basidiomycetes. The present study is the first investigation of endophytic wood fungi occurring in fir in Turkey using DNA-based methods. Therefore the study is expected to provide new information of the pioneering colonizers of wood tissues in stems of living Taurus firs.
