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Öğe Ceratocystis platani is killing plane trees in Istanbul (Turkey)(Wiley, 2018) Lehtijaervi, Asko Tapio; Oskay, F.; Lehtijarvi, H. T. Dogmus; Kaya, A. G. Aday; Pecori, F.; Santini, A.Ceratocystis platani was detected at several locations in the European side of Istanbul, causing severe dieback and mortality, mainly on Platanusxacerifolia imported from Italy approximately 160years ago, but also on Platanus orientalis. In the work reported here, the causal agent of the dieback and mortality was identified based on morphological characteristics in culture and internal transcribed spacer (ITS) sequencing, confirming the presence of C.platani in Turkey. All analysed C.platani isolates from Istanbul shared the same ITS sequence with European C.platani strains available in the NCBI, but differed from other species previously ascribed to the Ceratocystis fimbriata species complex. Pathogenicity of C.platani was proven by inoculation onto 25-cm-long and 4-9mm diam. P.orientalis twigs and the causal agent reisolated from symptomatic tissues. In a survey of 976 Platanus in streets and parks of Istanbul, 26.5% of trees showed symptoms of C.platani infections, whilst 5.6% were dead. The proportion of symptomatic trees was higher where management interventions, such as topping or pruning and felling infected trees had been carried out.Öğe Oomycota species associated with deciduous and coniferous seedlings in forest tree nurseries of Western Turkey(Wiley, 2017) Lehtijaervi, Asko Tapio; Kaya, A. G. Aday; Woodward, S.; Jung, T.; Lehtijarvi, H. T. DogmusOccurrence and pathogenicity of Oomycota species causing root rot were investigated in 10 forest tree nurseries in western Turkey. Soil samples (129 in total) taken from the rhizosphere of symptomatic seedlings were baited for Oomycota using young leaves of Quercus suber, Rhododendron simsii and R.ponticum. Oomycota (178 isolates) were obtained by culturing on selective media, and identified using morphological methods followed by PCR and sequencing of the ITS rDNA and cox1 regions. Phytophthora aff. cactorum, P.citricola sensu lato, P.crassamura, P.syringae, Pythium aphanidermatum, Py.intermedium, Py.irregulare, Py.ultimum and Phytopythium vexans were common amongst the isolates. The highest diversity of Oomycota was found in the forest nurseries at Adapazar-Hendek and zmir-Torbal. Pathogenicity tests showed that the isolates caused lesions on a range of host plants. The importance of these nursery infections in transferring potentially damaging oomycete species to Turkish forests is discussed.