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    Heterobasidion Infection in Abies nordmanniana ssp. bornmulleriana Stands in Kastamonu Province
    (Kastamonu Univ, Orman Fak, 2012) Lehtijarvi, A.; Dogmus-Lehtijarvi, H. T.; Unal, S.; Karadeniz, M.; Kaya, A. G. Aday; Oskay, F.
    In this study, the proportion of Abies bornmulleriana trees infected by Heterobasidion spp. was investigated in Kastamonu Province in different types of stands. One 1 cm-thick disc was taken from each of 100 freshly cut trees, washed under a running tap, placed into plastic bags and incubated in growth chamber at 24 C degrees for 7 days. The area occupied by the conidial stage of Heterobasidion annosum s. l. was determined under a stereomicroscope using transparent film placed onto the upper surface of each disc. During this investigation conidia of Heterobasidion annosum s. l. were taken with a needle and placed onto agar plates. The obtained isolates were identified with pairing tests and DNA-based methods. All 36 isolates responded as Heterobasidion abietinum to the tester isolates. Identification based on PCR amplification with MJF - MJR and KJF-KFR primers gave the same result. 34% (34 out of 100) of the discs taken from the fir forests were found to be infected with H. abietinum. The characteristics of the colonized patches on the discs indicated that the H. abietinum colonies originated from stem infections of the trees. The proportion of the disc area covered by the conidial stage of the fungus was 80% in one of the discs, 6-10% in 6 discs, 1-5% in 18 discs, and 0-1 % in 11 discs. The conidiophores were observed in heartwood in only five samples.
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    Preliminary Results of Wood Endophytes of Abies cilicica in Yenisarbademli of Isparta Province
    (Kastamonu Univ, Orman Fak, 2012) Lehtijarvi, A.; Dogmus-Lehtijzarvi, H. T.; Kaya, A. G. Aday; Oskay, F.
    Fungal colonisation originating from endophytic thalli in wood of healthy Taurus fir (Abies cilicica) was studied. Bore cores were taken from living trees during field surveys. The bore cores were cut into 0.5 cm long fragments, washed with 70% (v/v) ethanol, flamed and placed into petri dishes containing malt extract, agar and streptomycin. The plates were incubated at 25 degrees C for 4 weeks. The outgrowing mycelia were pure-cultured and identified based on amplification and sequencing of the internal transcribed spacer (ITS) region of their nuclear ribosomal DNA. Totally 12 different fungal species were isolated from Abies cilicica wood cores. Five of them belong to Basidiomycetes. The present study is the first investigation of endophytic wood fungi occurring in fir in Turkey using DNA-based methods. Therefore the study is expected to provide new information of the pioneering colonizers of wood tissues in stems of living Taurus firs.