Yazar "Can, Gamze Dereli" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Evaluation of the cytotoxic and genotoxic/antigenotoxic effects of resveratrol in human limbal explant cultures
    (Springer, 2023) Can, Gamze Dereli; Taner, Gokce; Aydemir, Cigdem Inci
    Purpose Resveratrol (RSV) is a natural polyphenol phytoalexin compound and has long been considered to possess antioxidant and anti-inflammatory effects. In order to exploit the protective potential of RSV in anterior segment diseases, we investigated the possible cytotoxic, genotoxic/antigenotoxic effects of human limbal explant cultures to RSV and MMC or H2O2 alone and in combination. Methods A total of 18 limbal explant tissues obtained from three corneal donors were placed on the 12 well tissue culture polystyrene plates and cultured for 14 days. Cell growth from limbal explants was observed by inverted phase contrast microscopy. The cytotoxic effects of RSV was studied by neutral red uptake assay. For the assessment of the genotoxic and antigenotoxic effects, basic alkaline technique of comet assay was performed. Results It was found that the concentrations of RSV up to 100 mu M did not significantly affect the viability of outgrowth cells of limbal explant during 24 h exposure. When compared to negative control, all concentrations of RSV alone caused an increase in DNA strand breakage. Interestingly, 10 mu g/mL MMC alone caused similar tail intensity and tail moment values with RSV alone. On the other hand, RSV treatment in all doses seemed to decrease the DNA damage induced by either H2O2 or MMC. Conclusion RSV is an attractive natural compound for the purpose of oxidative stress reduction in ocular surface and can be utilized as a supplement to promote ocular surface regeneration in vivo.
  • Küçük Resim Yok
    Öğe
    Surface coatings of contact lenses for cell sheet transplantation
    (Wiley, 2024) Can, Gamze Dereli; Atay, Merve Tunca; Egil, Abdurrahim Can; Guney, Eda; Shemshad, Sepideh; Taner, Gokce; Ince, Gozde Ozaydin
    The field of corneal cell sheet engineering has evolved rapidly in the last decade; however, challenges related to transplantation into target tissue still exist, necessitating a new approach to integrate cell sheet technology onto a contact lens surface. In this study, a novel surface-modified contact lens was developed for corneal regenerative medicine to ensure the easy and safe integration of cell sheets into the target tissue. The lens surface was coated with a thermo-responsive polymer using the initiated chemical vapor deposition technique, facilitating the separation of tissue-integrated cell sheets from the coated lens surface by simply lowering the temperature. BEAS-2B and L929 cells, along with human limbal epithelial cells, were cultured on a crosslinked poly(2-(dimethylamino)ethyl methacrylate)-coated lens, providing temperature sensitivity for 14 days. At the end of the incubation period, the cultured cells were harvested as intact monolayer cell sheets through a simple temperature reduction, without enzymatic treatment or additional surgical procedures. Rapid and complete delamination of the cells planted and incubated on the coated lens surface was achieved at room temperature. Therapeutic contact lenses modified with cell sheet technology allowed targeted delivery of cells to the affected area of the cornea. Schematics depict the initated chemical vapor deposition of a temperature responsive polymer coating on contact lenses, enabling the temperature controlled detachment of limbal epithelial cells for cell sheet transplantation. image