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    INNER EAR HISTOLOGICAL FINDINGS OF INTRATYMPANIC APPLICATION OF ENCAPSULATED GEL-BASED GENTAMICIN
    (2024) Aslan, Gulay Guclu; Arslan, Ilker Burak; Yesil-Celiktas, Ozlem; Vatansever, Seda; Çukurova, İbrahim; Aslan, Asım; Kazan, Aslihan
    Introduction: In this study, histopathological changes in inner ear after intratympanic application of gentamicin prepared in encapsulated gel formulation were investigated. Materials and Methods: 200 ?l Poloxamer 407 solution containing 10 mg gentamicin in encapsulated form was prepared. Fourteen adult guinea pigs were used for the study. In Group A gentamicin in encapsulated gel formulation, in group B 200 ?l Poloxamer 407 solution, in Group C gentamicin alone were applied intratympanically to the right ears by 0.3 cc (6 mg) 3 times in a week. One week later following intratympanic applications animals were sacrificed and inner ears were dissected for histologic evaluation. In 4 animals, left ears were used as negative control group (Group D). All specimens were dyed with TUNEL method. Apoptotic cells were counted in the cochlea and lateral semicircular canal ampulla. Results: The percent of TUNEL positive cells was higher in Groups B and C comparing to Group A in organ of Corti, and higher in Groups B and C comparing to Group A in spiral ganglion. In ampulla of the lateral semicircular canal, the percentage was higher in Group C comparing to Groups A and B. Conclusion: Histologic destruction caused by gentamicin on the ampulla of the lateral semicircular canal was observed less in application of encapsulated form of gentamicin than in application of gentamicin alone. Destruction of the cells in the organ of Corti and the spiral ganglion in encapsulated form application was also less than gentamicin alone application.