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    Effect of Copper Oxide Nanoparticles on Prostate Cancer PC3 Cells
    (2023) Aksel, Mehran; Karaağaç, Dursun; Kaya, Refik Tolga; Ağırakar, Fatih; Kaya, Muhammed Buğra; Kızılcık, Pınar; Kuru, Alperen
    Copper oxide (CuO NPs) nanoparticles were synthesized by green synthesis and characterized by UV-Vis spectroscopy, FT-IR spectroscopy, SEM and EDX analysis. The shape of the CuO nanoparticles obtained is approximately spherical and their particle sizes range from 40 to 85 nm. According to the experimental results, two peaks at 305 and 318 nm in the 270-400 nm range in the UV-Vis absorption spectrum and one peak at 536 cm-1 in the FT-IR spectrum confirm the presence of copper oxide nanoparticles. In addition, the presence of biomolecules in aesculus hippocastanum(horse chestnut) extract on the surface of the copper oxide nanoparticles synthesized by green synthesis was determined by the peaks seen in the range of 3300 cm-1 - 1000 cm-1 in the FTIR spectrum. The images, shape and size of the copper oxide nanoparticles were determined by SEM, and the weight percent of the elements were determined by EDX. PC3 prostate cell lines were used in the study. After measuring the cytotoxic effect of the agents used in the study on prostate cancer cells, the apoptotic effect of this effect was determined by Hoechst/propidium iodide (HOPI) staining. Graphpad prism program was used to compare all parameters between groups, and \"one-way analysis of variance\" (one-way ANOVA) method and dunnet's test were applied to test whether there was any difference between at least two groups. It has been determined that the drug obtained from copper oxide nanoparticles has a cytotoxic effect on PC3 prostate cancer cells and this effect occurs through the apoptosis pathway.
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    Evaluating the Anticancer Potential of Green-Synthesized Silver/Silver Oxide and Copper/Copper Oxide Nanoparticles From Walnut Leaf Extract on Prostate Cancer Cells
    (Wiley-V C H Verlag Gmbh, 2025) Aksel, Mehran; Ozen, Murat; Karaagac, Dursun
    Green synthesis is a method used to obtain metal/metal oxide nanoparticles from plant roots, stems, and leaves. In this study, Ag/Ag2O and Cu/CuO nanoparticles (NPs) were synthesized by utilizing the reducing, stabilizing and capping properties of the extract obtained from walnut (Juglans regia) leaves. The characteristic properties of the synthesized NPs were determined using UV-visible spectrophotometry (UV-vis), fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), energy dispersive X-ray diffraction (EDX), and powder X-ray diffraction (XRD). SEM images showed that Ag/Ag2O and Cu/CuO NPs were spherical in shape, with particle diameters ranging from 15 to 85 nm. EDX analysis confirmed the presence of silver, copper, and oxygen elements. The cytotoxic activity of the green-synthesized NPs was evaluated in vitro against PC3 prostate cancer cells and L929 fibroblast cells. Ag/Ag2O and Cu/CuO NPs exhibited significant cytotoxic activity against PC3 cells but showed comparatively lower cytotoxicity toward L929 cells. In contrast, the walnut leaf extract alone did not exhibit cytotoxic activity. According to the results, the synthesized nanoparticles demonstrated a cytotoxic effect on PC3 prostate cancer cells, and this effect was mediated through apoptosis.