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    Chemical composition, antioxidant and antimicrobial activity of Colchicum speciosum Steven growing in Türkiye
    (University of Agriculture, 2022) Baltacı, Cemalettin; Öz, Mehmet; Fidan, Muhammed Said; Üçüncü, Osman; Karatas, şeyda Merve
    In the current study, the chemical constituents, antioxidant and antimicrobial activity of the volatile oil obtained from flowers and stems of C. speciosum growing in Türkiye were investigated. Both volatile oils were extracted through the hydrodistillation method in a Clevenger apparatus followed by the GC-MS analysis. The volatile oil yield attained from flowers and stems parts were determined as 0.62% and 0.65%, respectively. The most abundant compounds were determined in the flowers. Tricosane, octadecanal and hexadecanoic acid were identified to be the major components. Tricosane (38.28%) was defined to be the major component in flowers. 2-hydroxy benzaldehyde, tricosane and hexadecanoic acid were found to be the main compounds in the volatile oil of stems. 2-hydroxy benzaldehyde (46.49%) was found to be the main constituents in stems. According to the results antioxidant activity was attained as total antioxidant substance amount (86.77 mg AA/100 mL), total phenolic substance amount (65.64 mg GA/100 mL), total flavonoid substance amount (3.92 mg QE/100 mL), ferric (III) ion reducing antioxidant power capacity (6.88 mg FeSO4/100 mL) and free radical scavenging capacity (ABTS) (50.67 mg AA/100 mL) were obtained the highest antioxidant properties were obtained in flowers of C. speciosum. C. speciosum volatile oils had antimicrobial activity against Bacillus cereus in the flowers and had no activity against bacteria and yeast-mould in the stems. © 2022, University of Agriculture. All rights reserved.
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    Determination of chemical content and biological activities obtaining essential oil from Ribes petraeum Wulfen plant grown in Gümüşhane region
    (Gümüşhane Üniversitesi, 2022) Karataş, Şeyda Merve; Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemallettin; Üçüncü, Osman
    Essential oil is fragrant, volatile, aromatic oils mostly by hydrodistillation obtained from plants. Essential oil is an indispensable raw material source for the pharmaceutical, food and cosmetic industries. Gümüşhane region is very rich in terms of plant diversity. As a result of the field study, the plant that formed the basis of this article were collected from the growing place determined. In this study; essential oil of the leaves of R. petraeum plant were determined by hydrodistillation method in a Clevenger type device. The chemical compositions of volatile oil diluted with hexane were determined by analyzing with a GC-MS instrument. The amount of antioxidant activities of the obtained essential oil were determined by free radical scavenging (DPPH and ABTS) amounts, and Ferric (III) Reducing Antioxidant Power (FRAP) capacity according to methods. In addition, some bioactive compounds contents were determined by total phenolic content (TPC), total flavonoid content (TFC), total antioxidant activity (TAC) studies. Antimicrobial activities of volatile oil were attained against 23 different microorganisms by disc diffusion method. As a result; the percent essential oil yield was determined as 0.47% in the leaves of the R. petraeum. The chemical classes with the highest percentage of compounds in the essential oil of plant parts: Aldehydes (55.98%) were determined in the leaves of the R. petraeum. In addition, the main components in its essential oil was found as (E)-2-hexenal (52.90%) in the leaves of the R. petraeum. The determination of antioxidant activities obtained from samples collected from plant parts were determined as 18.73 mg AA/100 mL and 21.31 mg AA/100 mL in free radical scavenging amounts (DPPH and ABTS), and 6.50 mg FeSO4/100 mL in FRAP capacity. The bioactive component contents were found as 66.37 mg GAE/100 mL in total phenolic content (TPC), 1.51 mg QE/100 mL in total flavonoid content (TFC), 195.43 mg AA/100 mL in total antioxidant activity (TAC). According to the results of the antimicrobial activities analysis obtained from the collected sample of plant parts, leaves of R. petraeum were not showed any antimicrobial activity against the microorganisms used.