Ercelik, MelisTekin, CaglaParın, Fatma NurMutlu, BüşraDoğan, Hazal YılmazTezcan, GulcinAksoy, Secil AkGurbuz, MelisaYıldırım, KenanBekar, AhmetKocaeli, HasanTaskapilioglu, Mevlut OzgurEser, PinarTunca, Berrin2024-06-062024-06-062023Ercelik M, Tekin C, Parin FN, Mutlu B, Dogan HY, Tezcan G, Aksoy SA, Gurbuz M, Yildirim K, Bekar A, Kocaeli H, Taskapilioglu MO, Eser P, Tunca B. Co-loading of Temozolomide with Oleuropein or rutin into polylactic acid core-shell nanofiber webs inhibit glioblastoma cell by controlled release. Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126722. doi: 10.1016/j.ijbiomac.2023.126722. Epub 2023 Sep 8. PMID: 37673167.01418130https://hdl.handle.net/20.500.12885/2240Glioblastoma (GB) has susceptibility to post-surgical recurrence. Therefore, local treatment methods are required against recurrent GB cells in the post-surgical area. In this study, we developed a nanofiber-based local therapy against GB cells using Oleuropein (OL), and rutin and their combinations with Temozolomide (TMZ). The polylactic acid (PLA) core-shell nanofiber webs were encapsulated with OL (PLA), rutin (PLA), and TMZ (PLA) by an electrospinning process. A SEM visualized the morphology and the total immersion method determined the release characteristics of PLA webs. Real-time cell tracking analysis for cell growth, dual Acridine Orange/Propidium Iodide staining for cell viability, a scratch wound healing assay for migration capacity, and a sphere formation assay for tumor spheroid aggressiveness were used. All polymeric nanofiber webs had core-shell structures with an average diameter between 133 ± 30.7-139 ± 20.5 nm. All PLA webs promoted apoptotic cell death, suppressed cell migration, and spheres growth (p < 0.0001). PLA and PLA suppressed GB cell viability with a controlled release that increased over 120 h, while PLA caused rapid cell inhibition (p < 0.0001). Collectively, our findings suggest that core-shell nano-webs could be a novel and effective therapeutic tool for the controlled release of OL and TMZ against recurrent GB cells.eninfo:eu-repo/semantics/closedAccessCombined treatmentGlioblastoma local treatmentNano-based drug delivery systemsArticlehttps://doi.org/10.1016/j.ijbiomac.2023.126722253WOS:00107775110000137673167Q1Q1